Volume 16, Issue 3 (Autumn 2009)                   J Birjand Univ Med Sci. 2009, 16(3): 9-15 | Back to browse issues page

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Serati Nouri H, Khayat Nouri M, Safavi S, Khaki A. Histological and spermatogenesis indexes study of testis after Methotrexate administration in adult male rat. J Birjand Univ Med Sci.. 2009; 16 (3) :9-15
URL: http://journal.bums.ac.ir/article-1-481-en.html
1- Department of Pharmacology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch , khayat_nouri@yahoo.com
2- Department of Pharmacology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch
Abstract:   (22836 Views)

  Background and Aim: Methotrexate (MTX) as a chemotherapeutic agent is used for the treatment of a variety of tumors and inflammatory diseases. Different studies have shown that methotrexate has inhibitory effects on oogenesis and spermatogenesis, and causes impair fertility and different pathological changes in testis of laboratory animals. The aim of this study is to investigate the effect of methotrexate administration on spermatogenesis indexes in the testis of adult male rats.

  Materials and Methods: In this experimental study, 40 male Wistar rats were randomly divided into experimental (n=20) and control (n=20). The first and the second experimental groups were received MTX (1 mg/kg, IP) per week for two and four consequence week, respectively. The first and second control groups just received normal saline (10 ml/kg, IP) per week for two and four consequence weeks, respectively. Tissue sampling of the testis was done after the mentioned time for each group, tissue slices were stained by H&E technique. Histological and spermatogenesis indexes which include: tubular differentiation index (TDI), spermiogenesis index (SI), and repopulation index (RI), were studied. Data were analyzed by ANOVA at the significant level of P<0.05.

  Results: This study showed that methotrexate had destructive effects on testis tissue and spermatogenesis in treated group compared with the control. Also, results showed that TDI, RI and SI had non significant decreased in 14th day, but in 28th day after treatment with MTX, all indexes were significantly decreased compared with control group (P<0.001).

Conclusion: This study showed that methotrexate administration (for 28 days, once a week) causes reduction of spermatogenesis and its indexes in rat testis tissue, probably due to reducing of cell divide and toxic effect of MTX. However, more studies are needed, to investigate the effect of this drug on spermatogenesis and infertility in human.
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Type of Study: Original Article | Subject: Histology
Received: 2009/10/12 | Accepted: 2016/03/10 | ePublished: 2016/03/10

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