The effect of MSN-NH2-curcumin in altering the expression pattern of apoptosis genes in RPMI-8226 multiple myeloma cell line

Nasiri, Maziar; Sayadi, Mahtab; Shakibaie, Mehdi; Sadooghi Rad, Elias; Anani Sarab, Gholamreza

Volume 33, Issue 1 (Articles In Press 2026) J Transl Med Res. 2026, 33(1): 0-0 | Back to browse issues page

Research code: 457368

Ethics code: IR.BUMS.REC.1402.553

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Nasiri M, Sayadi M, Shakibaie M, Sadooghi Rad E, Anani Sarab G. The effect of MSN-NH2-curcumin in altering the expression pattern of apoptosis genes in RPMI-8226 multiple myeloma cell line. J Transl Med Res. 2026; 33 (1)
URL: http://journal.bums.ac.ir/article-1-3602-en.html

The effect of MSN-NH2-curcumin in altering the expression pattern of apoptosis genes in RPMI-8226 multiple myeloma cell line

Maziar Nasiri¹

, Mahtab Sayadi²

, Mehdi Shakibaie³

, Elias Sadooghi Rad¹

, Gholamreza Anani Sarab ^*⁴

1- Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
2- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
3- Pharmaceutical Sciences Research Center, Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran
4- Department of Immunology, Birjand University of Medical Sciences, Birjand, Iran , ghansa@yahoo.com

Abstract: (160 Views)

Background and Aims: Multiple myeloma is an incurable plasma cell hematologic malignancy associated with dysregulation of the apoptotic process. The present study aimed to evaluate the effect of Curcumin-Loaded Amine-Functionalized Mesoporous Silica Nanoparticles (MSNs-NH₂-Cur) on the expression pattern of key apoptosis-related genes in the RPMI-8226 multiple myeloma cell line.
Materials and Methods: In this experimental study, RPMI-8226 cells were treated with MSNs-NH₂-Cur at the previously determined IC₅₀ concentration for 24 hours. Subsequently, PCR efficiency was calculated using LinRegPCR software, and the relative mRNA expression levels of p53, Bax, Bcl-2, and Beclin-1 were quantified and analyzed by quantitative real-time PCR (qRT-PCR).
Results: According to the findings, MSNs-NH₂-Cur treatment resulted in a significant upregulation of TP53 at the transcriptional level (P<0.01). In contrast, no significant changes were observed in Beclin-1 expression or in the Bax/Bcl-2 ratio (P>0.05). These findings suggested the activation of a p53-dependent response in this cellular model.
Conclusion: The results of the study demonstrated that MSNs-NH₂-Cur significantly upregulated TP53 gene expression, whereas no notable changes were observed in mitochondrial or autophagy-related markers. These findings also indicated the activation of the p53 pathway; however, further validation at the protein level and additional follow-up studies are required to substantiate these results.

Keywords: Apoptosis, p53, Curcumin-loaded amine-functionalized mesoporous silica nanoparticles, Multiple myeloma

Full-Text [PDF 519 kb] (40 Downloads)

Type of Study: Original Article | Subject: Hematology
Received: 2026/02/22 | Accepted: 2026/06/5 | ePublished: 2026/06/5

References

1. Malard F, Neri P, Bahlis NJ, Terpos E, Moukalled N, Hungria VTM, et al. Multiple myeloma. Nature Reviews Disease Primers. 2024;10(1):45. DOI: 10.1038/s41572-024-00529-7 [DOI:10.1038/s41572-024-00529-7] [PMID]

2. GCO. GLOBOCAN 2022, Islamic Republic of Iran fact sheet. 2022. Available online: https://gco.iarc.fr/today. [cited (accessed on 17 May 2022)].

3. Trtkova KS, Luzna P, Drozdkova DW, Cizkova K, Janovska L, Gursky J, et al. The epigenetic impact of suberohydroxamic acid and 5‑Aza‑2'‑deoxycytidine on DNMT3B expression in myeloma cell lines differing in IL‑6 expression. Mol Med Rep. 2022;26(4). DOI: 10.3892/mmr.2022.12837 [DOI:10.3892/mmr.2022.12837] [PMID] [PMCID]

4. Patiño-Morales CC, Soto-Reyes E, Arechaga-Ocampo E, Ortiz-Sánchez E, Antonio-Véjar V, Pedraza-Chaverri J, et al. Curcumin stabilizes p53 by interaction with NAD(P)H:quinone oxidoreductase 1 in tumor-derived cell lines. Redox Biol. 2020;28:101320. DOI: 10.1016/j.redox.2019.101320 [DOI:10.1016/j.redox.2019.101320] [PMID] [PMCID]

5. Iranshahy M, Hanafi-Bojd MY, Aghili SH, Iranshahi M, Nabavi SM, Saberi S, et al. Curcumin-loaded mesoporous silica nanoparticles for drug delivery: synthesis, biological assays and therapeutic potential - a review. RSC Adv. 2023;13(32):22250-67. DOI: 10.1039/D3RA02772D [DOI:10.1039/D3RA02772D] [PMID] [PMCID]

6. Egwu CO, Aloke C, Onwe KT, Umoke CI, Nwafor J, Eyo RA, et al. Nanomaterials in Drug Delivery: Strengths and Opportunities in Medicine. Molecules. 2024;29(11):2584. DOI: 10.3390/molecules29112584 [DOI:10.3390/molecules29112584] [PMID] [PMCID]

7. Xu B, Li S, Shi R, Liu H. Multifunctional mesoporous silica nanoparticles for biomedical applications. Signal Transduct Target Ther. 2023;8(1):435. DOI: 10.1038/s41392-023-01654-7 [DOI:10.1038/s41392-023-01654-7] [PMID] [PMCID]

8. AbouAitah K, Soliman AAF, Swiderska-Sroda A, Nassrallah A, Smalc-Koziorowska J, Gierlotka S, et al. Co-Delivery System of Curcumin and Colchicine Using Functionalized Mesoporous Silica Nanoparticles Promotes Anticancer and Apoptosis Effects. Pharmaceutics. 2022;14(12). DOI: 10.3390/pharmaceutics14122770 [DOI:10.3390/pharmaceutics14122770] [PMID] [PMCID]

9. Montazeri M, Pilehvar-Soltanahmadi Y, Mohaghegh M, Panahi A, Khodi S, Zarghami N, et al. Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines. Anticancer Agents Med Chem. 2017;17(5):662-73. DOI: 10.2174/1871520616666160815124537 [DOI:10.2174/1871520616666160815124537] [PMID]

10. Sinafar H, Noorbazargan H, Tafvizi F, Naseh V. Evaluation of apoptotic and anti-metastatic effects by modified mesoporous silica nanoparticles (MSNs) with Zn and NH2 containing cisplatin on HT-29 cell line. J NANOPART RES. 2023;25. DOI: 10.1007/s11051-023-05729-y [DOI:10.1007/s11051-023-05729-y]

11. Nasiri M, Anani Sarab G, Sayadi M, Shakibaie M. Effects of curcumin-loaded amine-functionalized mesoporous silica nanoparticles on apoptosis induction and cell cycle arrest in RPMI-8226 multiple myeloma cell line. Journal of Translational Medical Research. 2025;32(2):98-110. DOI: 10.61882/JBUMS.32.2.98 [DOI:10.61882/JBUMS.32.2.98]

12. Li W, Wang Y, Song Y, Xu L, Zhao J, Fang B. A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line. Oncol Lett. 2015;9(4):1719-24. DOI: 10.3892/ol.2015.2946 [DOI:10.3892/ol.2015.2946] [PMID] [PMCID]

13. Aubrey BJ, Kelly GL, Janic A, Herold MJ, Strasser A. How does p53 induce apoptosis and how does this relate to p53-mediated tumour suppression? Cell Death & Differentiation. 2018;25(1):104-13. DOI: 10.1038/cdd.2017.169 [DOI:10.1038/cdd.2017.169] [PMID] [PMCID]

14. Chen J. The Cell-Cycle Arrest and Apoptotic Functions of p53 in Tumor Initiation and Progression. Cold Spring Harb Perspect Med. 2016;6(3):a026104. DOI: 10.1101/cshperspect.a026104 [DOI:10.1101/cshperspect.a026104] [PMID] [PMCID]

15. Wu M, Ye H, Tang Z, Shao C, Lu G, Chen B, et al. p53 dynamics orchestrates with binding affinity to target genes for cell fate decision. Cell Death & Disease. 2017;8(10):e3130-e. DOI: 10.1038/cddis.2017.492 [DOI:10.1038/cddis.2017.492] [PMID] [PMCID]

16. Kong ZL, Kuo HP, Johnson A, Wu LC, Chang KLB. Curcumin-Loaded Mesoporous Silica Nanoparticles Markedly Enhanced Cytotoxicity in Hepatocellular Carcinoma Cells. Int J Mol Sci. 2019;20(12). DOI: 10.3390/ijms20122918 [DOI:10.3390/ijms20122918] [PMID] [PMCID]

17. M G, Y L, Z L, M Z, M X, C W, et al. Surface decoration of selenium nanoparticles with curcumin induced HepG2 cell apoptosis through ROS mediated p53 and AKT signaling pathways. RSC Adv. 2017;7:52456-64. DOI: 10.1039/C7RA08796A [DOI:10.1039/C7RA08796A]

18. Shanmugam MK, Rane G, Kanchi MM, Arfuso F, Chinnathambi A, Zayed ME, et al. The Multifaceted Role of Curcumin in Cancer Prevention and Treatment. Molecules. 2015;20(2):2728-69. DOI: 10.3390/molecules20022728 [DOI:10.3390/molecules20022728] [PMID] [PMCID]

19. Mohebian Z, Babazadeh M, Zarghami N. In Vitro Efficacy of Curcumin-Loaded Amine-Functionalized Mesoporous Silica Nanoparticles against MCF-7 Breast Cancer Cells. Adv Pharm Bull. 2023;13(2):317-27 . DOI: 10.34172/apb.2023.035 [DOI:10.34172/apb.2023.035] [PMID] [PMCID]

20. Sharifi S, Dalir Abdolahinia E, Ghavimi MA, Dizaj SM, Aschner M, Saso L, et al. Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5. Curr Issues Mol Biol. 2022;44(11):5247-59. DOI: 10.3390/cimb44110357 [DOI:10.3390/cimb44110357] [PMID] [PMCID]

21. Mekkawy SA, Abdalla MS, Omran MM, Hassan NM, Abdelfattah R, Abdel-Salam IM. Cancer Stem Cells as a Prognostic Biomarker and Therapeutic Target Using Curcumin/ Piperine Extract for Multiple Myeloma. Asian Pac J Cancer Prev. 2022;23(10):3507-15. DOI: 10.31557/APJCP.2022.23.10.3507 [DOI:10.31557/APJCP.2022.23.10.3507] [PMID] [PMCID]

22. Ahmadi A, Sokunbi M, Patel T, Chang MW, Ahmad Z, Singh N. Influence of Critical Parameters on Cytotoxicity Induced by Mesoporous Silica Nanoparticles. Nanomaterials (Basel). 2022;12(12). DOI: 10.3390/nano12122016 [DOI:10.3390/nano12122016] [PMID] [PMCID]

23. Sadat Razavi M, Ahmadi F, Ebrahimnejad P, Akbarzadeh A, Farokhrou M, Nokhodchi A. Harnessing Nanotechnology for Optimized Herbal Cancer Treatment: A Comprehensive Review of Nanoscale Drug Delivery Systems. Pharm Sci. 2024;30(2):153-86. DOI: 10.34172/PS.2023.27 [DOI:10.34172/PS.2023.27]

24. Atmaca H, Oguz F, Ilhan S. Chitosan in cancer therapy: a dual role as a therapeutic agent and drug delivery system. Z Naturforsch C J Biosci. 2024;79(5-6):95-105. DOI: 10.1515/znc-2023-0148 [DOI:10.1515/znc-2023-0148] [PMID]