Volume 33, Issue 1 (Articles In Press 2026)
J Transl Med Res. 2026, 33(1): 0-0 |
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Research code: 457368
Ethics code: IR.BUMS.REC.1402.553
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Nasiri M, Sayadi M, Shakibaie M, Sadooghi Rad E, Anani Sarab G. Investigating the effect of MSN-NH2-curcumin in altering the expression pattern of apoptosis genes in RPMI-8226 multiple myeloma cell line. J Transl Med Res. 2026; 33 (1)
URL: http://journal.bums.ac.ir/article-1-3602-en.html
URL: http://journal.bums.ac.ir/article-1-3602-en.html
1- , ghansa@yahoo.com
Abstract: (7 Views)
Background and Aims: Multiple myeloma is an incurable plasma cell hematologic malignancy characterized by dysregulation of the apoptotic process. The present study aimed to evaluate the effect of curcumin-loaded amine-functionalized mesoporous silica nanoparticles (MSNs-NH₂-Cur) on the expression pattern of key apoptosis-related genes in the RPMI-8226 multiple myeloma cell line.
Materials and Methods: In this experimental study, RPMI-8226 cells were treated for 24 hours with MSNs-NH₂-Cur at the previously determined IC₅₀ concentration. PCR efficiency was calculated using LinRegPCR software, and the relative mRNA expression levels of P53, Bax, Bcl-2, and Beclin-1 were quantified and analyzed by quantitative real-time PCR (qRT-PCR).
Results: MSNs-NH₂-Cur treatment resulted in a significant upregulation of TP53 at the transcriptional level (P < 0.01). In contrast, no significant changes were observed in Beclin-1 expression or in the Bax/Bcl-2 ratio (P > 0.05). These findings suggest activation of a p53-dependent response in this cellular model.
Conclusion: The study demonstrated that MSNs-NH₂-Cur significantly upregulated TP53 gene expression, whereas no notable changes were observed in mitochondrial or autophagy-related markers. These findings indicate activation of the p53 pathway and warrant further validation at the protein level and additional follow-up studies.
Materials and Methods: In this experimental study, RPMI-8226 cells were treated for 24 hours with MSNs-NH₂-Cur at the previously determined IC₅₀ concentration. PCR efficiency was calculated using LinRegPCR software, and the relative mRNA expression levels of P53, Bax, Bcl-2, and Beclin-1 were quantified and analyzed by quantitative real-time PCR (qRT-PCR).
Results: MSNs-NH₂-Cur treatment resulted in a significant upregulation of TP53 at the transcriptional level (P < 0.01). In contrast, no significant changes were observed in Beclin-1 expression or in the Bax/Bcl-2 ratio (P > 0.05). These findings suggest activation of a p53-dependent response in this cellular model.
Conclusion: The study demonstrated that MSNs-NH₂-Cur significantly upregulated TP53 gene expression, whereas no notable changes were observed in mitochondrial or autophagy-related markers. These findings indicate activation of the p53 pathway and warrant further validation at the protein level and additional follow-up studies.
Keywords: Apoptosis, p53, Curcumin-loaded amine-functionalized mesoporous silica nanoparticles, Multiple myeloma
Type of Study: Original Article |
Subject:
Hematology
Received: 2026/02/22 | Accepted: 2026/06/5 | ePublished: 2026/06/5
Received: 2026/02/22 | Accepted: 2026/06/5 | ePublished: 2026/06/5
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