Effects of curcumin-loaded amine-functionalized mesoporous silica nanoparticles on apoptosis induction and cell cycle arrest in RPMI-8226 multiple myeloma cell line

Nasiri, Maziar; Anani Sarab, Gholamreza; Sayadi, Mahtab; Shakibaie, Mehdi

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Research code: 457368

Ethics code: IR.BUMS.REC.1402.553

Effects of curcumin-loaded amine-functionalized mesoporous silica nanoparticles on apoptosis induction and cell cycle arrest in RPMI-8226 multiple myeloma cell line

Maziar Nasiri¹

, Gholamreza Anani Sarab²

, Mahtab Sayadi²

, Mehdi Shakibaie ^*³

1- Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
2- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
3- Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran & Pharmaceutical Sciences Research Center, Birjand University of Medical Sciences, Birjand, Iran , m.shakibaie@bums.ac.ir

Abstract: (21 Views)

Background and Aims: Multiple Myeloma (MM) is an incurable hematologic malignancy characterized by the dysregulation of apoptosis and cell cycle processes. The pressent study aimed to investigate the effects of curcumin-loaded amine-functionalized mesoporous silica nanoparticles (MSNs-NH₂-Cur) on apoptosis induction and cell cycle phase alterations in RPMI-8226 multiple myeloma cell.
Materials and Methods: In this experimental study, MSNs were synthesized using modified Stöber method and subsequently functionalized with amine groups. The synthesized nanoparticles were characterized using Transmission electron microscopy (TEM) images and Dynamic Light Scattering (DLS) analysis. MSNs-NH₂ were then loaded with curcumin, and the percentages of encapsulation efficiency and loading efficiency were measured. RPMI-8226 cells were cultured in RPMI-1640 media and their viability was assessed after 24- and 48-hour treatments with various concentrations of MSNs-NH₂-Cur using the MTT assay. Following 24-hour treatment of RPMI-8226 cells with IC₅₀ concentration, cell cycle distribution was examined through Propidium iodide staining, and apoptosis was evaluated using the Annexin V-PI kit via flow cytometry.
Results: TEM images and DLS analysis demonstrated the morphology and uniform size distribution of the nanoparticles. The loading efficiency was relatively satisfactory; moreover, a slow and sustained release of curcumin from MSN-NH₂ was observed. MSNs-NH₂-Cur reduced the viability of RPMI-8226 cells in a concentration- and time-dependent manner (P˂ 0.001). Flow cytometric analysis clearly illustrated cell cycle arrest in the G₀/G₁ phase (32.276% to 46.233%) (P˂ 0.01) and induction of apoptosis.

Conclusion: As evidenced by the obtained results, MSNs-NH₂-Cur significantly decreased cell viability in a concentration- and time-dependent manner, somewhat induced apoptosis, and caused cell cycle arrest.

Keywords: Apoptosis, Cell cycle, Curcumin-loaded amine-functionalized mesoporous silica nanoparticles, Multiple myeloma

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Type of Study: Original Article | Subject: Nanotechnology
Received: 2025/06/25 | Accepted: 2025/07/28 | ePublished ahead of print: 2025/08/25