Volume 30, Issue 1 (June 2023)
J Birjand Univ Med Sci. 2023, 30(1): 99-106 |
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Research code: 6318
Ethics code: IR.BUMS.REC.1402.156
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Zangooei M, Bagheri V. Simultaneous effect of progesterone and berberine on the level of reactive oxygen species in K562 cell. Journals of Birjand University of Medical Sciences 2023; 30 (1) :99-106
URL: http://journal.bums.ac.ir/article-1-3241-en.html
URL: http://journal.bums.ac.ir/article-1-3241-en.html
1- Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
2- Cellular and Molecular Research Center, Department of Medical Immunology, Birjand University of Medical Sciences, Birjand, Iran ,eftekhar2006@gmail.com
2- Cellular and Molecular Research Center, Department of Medical Immunology, Birjand University of Medical Sciences, Birjand, Iran ,
Abstract: (701 Views)
Chronic myeloid leukemia (CML) is an uncommon type of white blood cells cancer that originates from bone marrow stem cells. Progesterone (P4) and berberine (BBR) are bioactive compounds that inhibit the growth of tumor cells. The present study aimed to assess the simultaneous effect of P4 and BBR on the level of reactive oxygen species (ROS) in K562 cells. The K562 cells were cultured in a complete cell culture medium and simultaneously exposed to IC50 different concentrations of P4 and BBR at 24 h (P4:102.4 μM; BBR:125 μM), 48 h (P4:78.4 μM; BBR:114 μM), and 72 h (P4:70 μM; BBR:45 μM). Then, the cell viability and cellular ROS level were determined using MTT assay and 2′, 7′-dichlorofluorescin diacetate (DCF) by flow cytometry, respectively. Our results showed that the combination of P4 and BBR inhibited the cells more effectively than P4 and BBR alone at 72 h, as well as P4 and their combination reduced ROS level in the cells compared to BBR and untreated cells at 24 h and 72 h. Taken together, P4 through a ROS-dependent pathway and BBR through a ROS-independent pathway may be inhibited cell growth.
*Corresponding Author: Vahid Bagheri; Emails: eftekhar2006@gmail.com
*Corresponding Author: Vahid Bagheri; Emails: eftekhar2006@gmail.com
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Keywords: Berberine, Cell survival, Berberine, CML, K562 cells, Progesterone, Reactive oxygen species
Type of Study: Short Communication |
Subject:
Oncology
Received: 2023/01/27 | Accepted: 2023/05/1 | ePublished ahead of print: 2023/05/3 | ePublished: 2023/06/5
Received: 2023/01/27 | Accepted: 2023/05/1 | ePublished ahead of print: 2023/05/3 | ePublished: 2023/06/5
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13. Wang Y, Liu Y, Du X, Ma H, Yao J. The Anti-Cancer Mechanisms of Berberine: A Review. Cancer Manag Res. 2020; 12: 695-702.
14. Li Z, Jiang T, Lu Q, Xu K, He J, Xie L, et al. Berberine attenuated the cytotoxicity induced by t-BHP via inhibiting oxidative stress and mitochondria dysfunction in PC-12 cells. Cell Mol Neurobiol. 2020: 40(4): 587-602.
15. Zhang R, Qiao H, Chen S, Chen X, Dou K, Wei L, et al. Berberine reverses lapatinib resistance of HER2-positive breast cancer cells by increasing the level of ROS. Cancer Biol Ther. 2016; 17(9): 925-34.
16. Radivoyevitch T, Jankovic GM, Tiu RV, Saunthararajah Y, Jackson RC, Hlatky LR, et al. Sex differences in the incidence of chronic myeloid leukemia. Radiat Environ Biophys. 2014; 53(1): 55-63.
17. Druker BJ. Translation of the Philadelphia chromosome into therapy for CML. Blood. 2008; 112(13): 4808-17.
18. Antoszewska-Smith J, Pawlowska E, Blasiak J. Reactive oxygen species in BCR-ABL1-expressing cells - relevance to chronic myeloid leukemia. Acta Biochim Pol. 2017; 64(1): 1-10.
19. Nguyen H, Syed V. Progesterone inhibits growth and induces apoptosis in cancer cells through modulation of reactive oxygen species. Gynecol Endocrinol. 2011; 27(10): 830-6.
20. Zhang C, Sheng J, Li G, Zhao L, Wang Y, Yang W, et al. Effects of Berberine and Its Derivatives on Cancer: A Systems Pharmacology Review. Front Pharmacol. 2019; 10: 1461.
21. Habtemariam S. Recent Advances in Berberine Inspired Anticancer Approaches: From Drug Combination to Novel Formulation Technology and Derivatization. Molecules. 2020; 25(6).
22. Tong L, Chuang CC, Wu S, Zuo L. Reactive oxygen species in redox cancer therapy. Cancer Lett. 2015; 367(1): 18-25.
23. Zou Z, Chang H, Li H, Wang S. Induction of reactive oxygen species: an emerging approach for cancer therapy. Apoptosis. 2017; 22(11): 1321-35.
24. Pascu VEG, AM GA. Assessment of Oxidative Stress in Patients with Chronic Myeloid Leukemia Depending on Associated Comorbidities. Curr Health Sci J. 2020; 46(1): 23-30. DOI: 10.12865/CHSJ.46.01.04
25. Fink M. Possible effect of medroxyprogesterone acetate (MPA) in lymphoid blast crisis of chronic myelogenous leukemia. Ann Hematol. 1994; 68(2): 89-90.
26. Achi IT, Sarbadhikary P, George BP, Abrahamse H. Multi-Target Potential of Berberine as an Antineoplastic and Antimetastatic Agent: A Special Focus on Lung Cancer Treatment. Cells. 2022; 11(21): 3433.
27. Wassmann K, Wassmann S, Nickenig G. Progesterone antagonizes the vasoprotective effect of estrogen on antioxidant enzyme expression and function. Circ Res. 2005; 97(10): 1046-54.
28. Wang Y, Liu Y, Du X, Ma H, Yao J. The Anti-Cancer Mechanisms of Berberine: A Review. Cancer Manag Res. 2020; 12: 695-702.
29. Li Z, Jiang T, Lu Q, Xu K, He J, Xie L, et al. Berberine attenuated the cytotoxicity induced by t-BHP via inhibiting oxidative stress and mitochondria dysfunction in PC-12 cells. Cell Mol Neurobiol. 2020: 40(4): 587-602.
30. Zhang R, Qiao H, Chen S, Chen X, Dou K, Wei L, et al. Berberine reverses lapatinib resistance of HER2-positive breast cancer cells by increasing the level of ROS. Cancer Biol Ther. 2016; 17(9): 925-34.
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