Volume 29, Issue 3 (October 2022)
J Birjand Univ Med Sci. 2022, 29(3): 218-228 |
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Research code: IR.SHAHED.REC.1399.074
Ethics code: IR.SHAHED.REC.1399.074
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Jasbinia F, Hassanpourezatti M. Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats. Journals of Birjand University of Medical Sciences 2022; 29 (3) :218-228
URL: http://journal.bums.ac.ir/article-1-3191-en.html
URL: http://journal.bums.ac.ir/article-1-3191-en.html
1- Master of Science, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ,hassanpour@shahed.ac.ir
2- Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ,
Abstract: (860 Views)
Background and Aims: Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CMs on the thermal treatment of reserpine-induced FM and evaluate the concentration changes of tumor necrosis factor-alpha (TNF-α) in the hippocampus of rats under these conditions.
Materials and Methods: 220 g. Animals received reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0.3 and 0.5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α concentration in the hippocampus was determined by an ELISA kit at the end of the experimental period.
Results: The thermal pain threshold of rats in the FM group decreased significantly (P<0.01) in both pain tests compared to that of the control group. A significant increase (P<0.01) was observed in the level of TNF-α in the hippocampus of rats in the FM group compared to that of the control group. Treatment with MSC-CMs significantly reduced reserpine-induced pain and hippocampal TNF-a concentration despite the effect of fresh and conditioned medium on control rats.
Conclusion: Treatment with MSC-CMs could control thermal pain in the rat model of reserpine-induced fibromyalgia by controlling the TNF-a level in the hippocampus.
Materials and Methods: 220 g. Animals received reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0.3 and 0.5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α concentration in the hippocampus was determined by an ELISA kit at the end of the experimental period.
Results: The thermal pain threshold of rats in the FM group decreased significantly (P<0.01) in both pain tests compared to that of the control group. A significant increase (P<0.01) was observed in the level of TNF-α in the hippocampus of rats in the FM group compared to that of the control group. Treatment with MSC-CMs significantly reduced reserpine-induced pain and hippocampal TNF-a concentration despite the effect of fresh and conditioned medium on control rats.
Conclusion: Treatment with MSC-CMs could control thermal pain in the rat model of reserpine-induced fibromyalgia by controlling the TNF-a level in the hippocampus.
Type of Study: Original Article |
Subject:
Physiology
Received: 2022/07/14 | Accepted: 2022/12/5 | ePublished ahead of print: 2022/12/6 | ePublished: 2022/12/6
Received: 2022/07/14 | Accepted: 2022/12/5 | ePublished ahead of print: 2022/12/6 | ePublished: 2022/12/6
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