Volume 21, Issue 2 (Summer 2014)                   J Birjand Univ Med Sci. 2014, 21(2): 160-168 | Back to browse issues page

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Daneshmandi S, Pourfathollah A A, Forouzandeh-Moghaddam M. Chitosan nanopolymers effect in activating of mouse bone marrow derived dendritic cells. J Birjand Univ Med Sci.. 2014; 21 (2) :160-168
URL: http://journal.bums.ac.ir/article-1-1465-en.html
1- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;
2- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , Pourfa@modares.ac.ir
3- Department of Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (9697 Views)

Background and Aim: Regarding to various problems in the activation of dendritic cells and immune system’s responses, finding of a safe, effective and applicable agent is highly desirable. Chitosan is an effective gene delivery agent and also a part of nanoscaffolds. In the present study,chitosan nanopolymers effect on dendritic immune cells were assessed. Materials and Methods: In this experimental-laboratory study, chitosan (150 KD) in acetic acid 1% solution was depolymerized to 10 KD oligomers using NaNO2. The oligomers particles were obtained by means of 2 normal NAOH molecules. Denderitic cells were derived from the rats’ bone marrow using GM-SCF. On the treated denderitic cells CD40, CD86 and MHC-II maturation markers were evaluated by flowcytometery and TNF-α release was evaluated using ELISA method and T cell proliferation. Results: It was observed that dendrtic cells purity on the 8th day was more than 90%. Flowcytometery analysis showed an increase in all evaluated CD40, CD86 and MHC-II maturation markers (p<0.05). TNF-α release and T cell proliferation significantly increased by chitosan treated denderitic cells compared to unstimulated or lipofectamin treated cells (P<0.05). Conclusion: Results showed that chitosan nanopolymers significantly increased dendertic cell maturation phenotype, proinflamatory cytokine production, and induction of T cell proliferation. Therefore, chitosan nanocomplexes and scaffolds can induce and accelerate immune responses.

Full-Text [PDF 447 kb]   (1762 Downloads)    
Type of Study: Original Article | Subject: Immunology
Received: 2013/07/31 | Accepted: 2013/12/30 | ePublished: 2014/09/4

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