Volume 13, Issue 2 (Summer 2006)                   J Birjand Univ Med Sci. 2006, 13(2): 9-15 | Back to browse issues page

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Ghaderi R, MalekiNejad P. Evaluation of anticandidial effects of Berberis vulgaris root extracts (ethanolic and aqueous) and comparing their effects with those of clotrimazole. J Birjand Univ Med Sci.. 2006; 13 (2) :9-15
URL: http://journal.bums.ac.ir/article-1-88-en.html
1- Associate Professor , rezaghaderi@yahoo.com
2- Student of Medicine
Abstract:   (18614 Views)
Background and Aim: During the past three decades, life-threatening fungal infections have frequently been emerging through opportunistic infections with the advent of cancer chemotherapy, organ transplantation, and or AIDS. Most of systemic fungal infections are mainly caused by opportunistic pathogens such as Candida species. Although many researchers have carried out intensive studies attempting to develop new antifungal drugs some of which are now under clinical trials, amphotericin B and the azole compounds remain the mainstay of fungal systemic infection therapy. However, the adverse effects of polyenes and the emergence of Candida strains resistant to commercial azole compounds make the treatment of patients with deeply invasive mycoses difficult. Therefore, demand for the development of new antifungal agents which have low side effects and broad-spectrum activity against various fungi is greater than ever before. The present study aimed at evaluating the anticandidial effects of Berberis vulgaris Root extracts (ethanolic and aqueous) and comparing their effects with those of clotrimazole.
Materials and Methods: In this experimental study, aqueous and ethanolic extracts of Berberis each having 1 mg/mL concentration were prepared. Candida albicans (100000 CFU/mL) was cultured on dextrose agar medium .Then, after incubation period of 48 hours, anticandidal strength of the extracts and clotrimazole was measured through zone of inhibition. Zone of inhibition for 1-10 mm was 1+, for 10-20 mm 2+, for 20 –30 mm 3+ and for 30-40 mm 4+. The obtained data was analysed by using SPSS software package, ANOVA and Tukey B tests.
Resutls: Average zone of inhibition for aqueous extract of Berberis was 16.89 mm, for ethanolic extract of Berberis it was 27.69 mm, for pure ethanol 0 mm, and for clotrimazole 33.63 mm. Besides, these differences were statistically significant (P<0.001).
Conclusion: According to our findings, Berberis vulgaris root extracts have anticandidal effects, and these effects are more prominent for ethanolic extract. Because ethanol, by itself, doesnchr('39')t have any anticandidal effects (zone of inhibition=0), we can conclude that all of the anticandidial effects of ethanolic extract of Berberis are due to Berberis vulgaris root extract.
Type of Study: Original Article | Subject: Mycology
Received: 2006/09/6 | Accepted: 2016/03/10 | ePublished: 2016/03/10

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