Volume 17, Issue 1 (April 2010)                   J Birjand Univ Med Sci. 2010, 17(1): 1-10 | Back to browse issues page

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Mansouri S, Shafiee-Nick R, Naghizadeh B, Parsaee H. Evaluation of the effects of new synthetic methylquinolinone derivatives on glucose-induced insulin secretion from rats' isolated Langerhans islets. J Birjand Univ Med Sci.. 2010; 17 (1) :1-10
URL: http://journal.bums.ac.ir/article-1-585-en.html
1- Assistant Professor, Department of Pharmacology, Physiology Research Center, Faculty of Medicine, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran , smt_mansouri@yahoo.com
Abstract:   (32826 Views)

  Background and Aim: Selective PDE3 inhibitors, via cyclic adenosine monophosphate (cAMP) accumulation increase cardiac contraction and augment glucose-induced insulin secretion. In this study, the effects of some synthetic methylquinolinone derivatives (MC1-MC10) on glucose-induced insulin secretion in rats' isolated Langerhans islets model were investigated.

  Materials and Methods: After the digestion of isolated pancreas using collagenase-IV, the isolated islets were collected manually under a stereomicroscope and were incubated in carboxyl buffer having 3mM glucose for 30 minutes. Then, they were incubated at 37°C presented to basal (3mM) and stimulatory (10mM) dose of glucose with or without different methylquinolinone derivatives and 3-isobutyl-1-methylxanthine (IBMX) (as standard) in 100µM concentration. After 60 minutes of incubation, the secreted insulin was measured using a radioimmunoassay method.

  Results: Glucose significantly increased insulin release with 10mM concentration in comparison with 3mM concentration (P<0.01). IBMX (100µM) significantly augmented glucose-induced insulin secretion (P<0.01). However, among the investigated ten compounds only MC7 and MC9 significantly increased glucose-induced insulin secretion (P<0.01) which was comparable with IBMX.

  Conclusion: In spite of having similar structure, the effect of the test compounds (MC1-MC10) on insulin secretion varied widely which may be due to their tissue-specific effects. Finally, it is hoped that the ligands will probably be used in the treatment of diabetes in the future.

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Type of Study: Original Article | Subject: Physiology
Received: 2010/03/8 | Accepted: 2016/03/10 | Published: 2016/03/10

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