Volume 24, Issue 3 (October 2017)                   J Birjand Univ Med Sci. 2017, 24(3): 170-178 | Back to browse issues page

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Rezaei Z, Kordi-Tamandani D M, Sebzari A, Dastjerdi K. Correlation between resistance to trastuzumab and miR-141 relative expression in the BT-474 human breast cancer cell line. J Birjand Univ Med Sci.. 2017; 24 (3) :170-178
URL: http://journal.bums.ac.ir/article-1-2325-en.html
1- Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran
2- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand
3- Cellular and Molecular Research Center, Birjand University of Medical Sciences , Birjnad , dastjerdi1974@hotmail.com
Abstract:   (3076 Views)

Background and Aim: Resistance to trastuzumab has been a critical barrier to targeted therapy of HER 2-positive (Human Epidermal Growth Factor Receptor 2) breast cancers. MicroRNAs (miRNAs) are known as decisive core regulators of drug resistance that modulate the epithelial-to-mesenchymal transition (EMT) and cancer-related immune responses. The present study aimed at examining the expression of miR-141 in trastuzumab-resistant and trastuzumab-sensitive BT-474 breast cancer cells.
Materials and Methods: In this experimental study, trastuzumab-resistant BT-474 cells were generated by continuous in-vitro culture of BT-474 cells in the presence of trastuzumab for six months. The relative expression of miR-141 to U6 RNA was then evaluated in trastuzumab-resistant and trastuzumab-sensitive cells using Relative Real-Time PCR. Mann-Whitney test was used to compare the difference between the two groups.
Results: There was a significant difference between the survival rates of resistant BT-474 cells and sensitive cells in the MTT test in the presence of different concentrations of trastuzumab showing that BT-474 breast cancer cells have turned resistant to this drug under long-term culture (P=0.001). Also, the expression of miR-141 in trastuzumab-resistant cells was significantly reduced by four times compared with the BT-474 parent cells (P=0.049).
Conclusion:  Down-regulation of miR-141 in trastuzumab-resistant BT-474 cells might be one possible mechanism for resistance against trastuzumab and an indication of the role of this microRNA in controlling the metastasis pathway.

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Type of Study: Original Article | Subject: Molecular Biology
Received: 2017/08/27 | Accepted: 2017/10/26 | Published: 2017/11/5

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