Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease

Haghighi, Arezoo; Salehi, Zivar; Aminian, Keyvan; Fakhrieh Asl, saba

Volume 21, Issue 1 (April 2014) J Birjand Univ Med Sci. 2014, 21(1): 48-55 | Back to browse issues page

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Haghighi A, Salehi Z, Aminian K, Fakhrieh Asl S. Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease. J Birjand Univ Med Sci. 2014; 21 (1) :48-55
URL: http://journal.bums.ac.ir/article-1-1438-en.html

Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease

Arezoo Haghighi¹, Zivar Salehi ^*², Keyvan Aminian³, Saba Fakhrieh Asl³

1- Cellular and Molecular Sciences, Department of Biology, Faculty of Guilan Pardis, Department of Biology, University of Guilan, Rasht, Iran.
2- Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran. , geneticzs@yahoo.co.uk
3- Gastroenterologist, Internal Medicine Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Abstract: (17837 Views)

Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species (ROS) in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation (OXPHOS) predisposes the deletion of 4977 bp mtDNA. The aim of the present study was to evaluate the association of 4977 bp mtDNA deletion with peptic ulcer disease. Materials and Methods: In this case-control study included 110 patients with peptic ulcer disease and 110 healthy individuals were compared. Genomic DNAs of the cases and controls were extracted from bioptic tissues. Then, their genotypes were determined by means of Polymerase Chain Reaction (PCR). Finally, statistical analysis was performed using the MedCalc program. Results: Deletion of 4977 bp mtDNA was found to be more frequent among patients with peptic ulcer disease (52.7%) compared to the controls (15.3%). A significant association was found between the deletion with peptic ulcer disease. Conclusion: Deletion of 4977 bp in mitochondrial DNA is associated with peptic ulcer disease.

Keywords: Peptic ulcer disease, Mitochondria genome

Full-Text [PDF 234 kb] (3274 Downloads)

Type of Study: Original Article | Subject: Medical Genetics
Received: 2013/06/26 | Accepted: 2014/03/13 | ePublished: 2014/06/10