Volume 33, Issue 3 (9-2026)
J Transl Med Res. 2026, 33(3): 175-189 |
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Mohammadi A, Mohammadi Mobarakeh S Z, Ansarifar E. Extending the shelf life of ground meat using active antimicrobial packaging. , J Transl Med Res.. 2026; 33 (3) :175-189
URL: http://journal.bums.ac.ir/article-1-3612-en.html
URL: http://journal.bums.ac.ir/article-1-3612-en.html
1- Department of Chemistry, Payame Nour University, Tehran, Iran
2- Department of Chemistry, Payame Nour University, Tehran, Iran ,Szmohammadi1396@gmail.com
3- Department of Food Hygiene and Nutrition, School of Health, Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran
2- Department of Chemistry, Payame Nour University, Tehran, Iran ,
3- Department of Food Hygiene and Nutrition, School of Health, Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran
Abstract: (602 Views)
Background and Aims: The rapid spoilage of minced meat due to lipid oxidation and microbial growth represents a major challenge in the food industry. This study aimed to develop an active packaging system based on electrospun polyvinyl alcohol/chitosan (PVA/CH) nanofibers incorporated with Tamarix extract (TREO) to extend the shelf life of minced beef during refrigerated storage (4∘C).
Materials and Methods: PVA/CH nanofibers, with and without 4% TREO, were fabricated via electrospinning and applied as an inner-lid coating of meat packaging. The samples were divided into three groups: control, PVA+CH, and PVA+CH+TREO. Microbial parameters, including total viable count (TVC) and psychrotrophic bacterial count (PTC), as well as physicochemical parameters, including pH, peroxide value (PV), total volatile basic nitrogen (TVB-N), free fatty acids (FFA), and thiobarbituric acid-reactive substances (TBARS), were evaluated on days 0, 2, 4, 6, 8, and 10.
Results: The control sample spoiled on day 6, exceeding permissible limits for chemical and microbial indicators (TVB−N=26.50 mg N/100g and TVC=8.35 log CFU/g). The application of PVA+CH nanofibers delayed the spoilage process until day 8. However, the extract-loaded treatment (PVA+CH+TREO) exhibited significantly superior performance (P<0.05). Owing to the synergistic antimicrobial and antioxidant effects of chitosan and TREO, alongside their controlled release, all quality attributes of the meat, including TVB−N (23.50 mg N/100g), TBARS (1.45 mg MDA/kg), and TVC (7.22 log CFU/g), remained within the acceptable consumption limits until the end of the 10th day.
Conclusion: Electrospun nanofibers incorporating TREO provide an effective matrix for mitigating oxidative and microbial spoilage, demonstrating considerable potential as an active, biodegradable packaging material for extending the shelf life of meat products.
Materials and Methods: PVA/CH nanofibers, with and without 4% TREO, were fabricated via electrospinning and applied as an inner-lid coating of meat packaging. The samples were divided into three groups: control, PVA+CH, and PVA+CH+TREO. Microbial parameters, including total viable count (TVC) and psychrotrophic bacterial count (PTC), as well as physicochemical parameters, including pH, peroxide value (PV), total volatile basic nitrogen (TVB-N), free fatty acids (FFA), and thiobarbituric acid-reactive substances (TBARS), were evaluated on days 0, 2, 4, 6, 8, and 10.
Results: The control sample spoiled on day 6, exceeding permissible limits for chemical and microbial indicators (TVB−N=26.50 mg N/100g and TVC=8.35 log CFU/g). The application of PVA+CH nanofibers delayed the spoilage process until day 8. However, the extract-loaded treatment (PVA+CH+TREO) exhibited significantly superior performance (P<0.05). Owing to the synergistic antimicrobial and antioxidant effects of chitosan and TREO, alongside their controlled release, all quality attributes of the meat, including TVB−N (23.50 mg N/100g), TBARS (1.45 mg MDA/kg), and TVC (7.22 log CFU/g), remained within the acceptable consumption limits until the end of the 10th day.
Conclusion: Electrospun nanofibers incorporating TREO provide an effective matrix for mitigating oxidative and microbial spoilage, demonstrating considerable potential as an active, biodegradable packaging material for extending the shelf life of meat products.
Type of Study: Original Article |
Subject:
Nutrition
Received: 2026/05/16 | Accepted: 2026/09/8 | ePublished: 2026/09/23
Received: 2026/05/16 | Accepted: 2026/09/8 | ePublished: 2026/09/23
References
1. Fattahian A, Fazlara A, Maktabi S, Pourmahdi broojeni M, Bavarsad N. The effects of edible chitosan coating containing Cuminum cyminum essential oil on the shelf-life of meat in modified atmosphere packaging. Journal of food science and technology (Iran). 2020;17(104):79-91. DOI: 10.52547/fsct.17.104.79 [DOI:10.52547/fsct.17.104.79]
2. Khan I, Tango CN, Oh D-H. Development and evaluation of chitosan and its derivative for the shelf life extension of beef meat under refrigeration storage. International Journal of food science and Technology. 2017;52(5):1111-21. DOI: 10.1111/ijfs.13379 [DOI:10.1111/ijfs.13379]
3. Karabagias I, Badeka A, Kontominas M. Shelf life extension of lamb meat using thyme or oregano essential oils and modified atmosphere packaging.Meat Sci. 2011;88(1):109-16. DOI: 10.1016/j.meatsci.2010.12.010 [DOI:10.1016/j.meatsci.2010.12.010] [PMID]
4. Rahman M, Haque E, Hasanuzzaman M, Shahid I. Antinociceptive, antiinflammatory and antibacterial properties of Tamarix indica roots. Int J Pharmacol. 2011;7(4):527-31. DOI: 10.3923/ijp.2011.527.531 [DOI:10.3923/ijp.2011.527.531]
5. Mohsenipour Z, Hassanshahian M. Inhibitory effects of Tamarix hispida extracts on planktonic form and biofilm formation of six pathogenic bacteria. Journal of Microbial Biology. 2015;4(13):43-56. [Persian] URL: https://bjm.ui.ac.ir/article_19565.html?lang=en
6. Boulaaba M, Snoussi M, Saada M, Mkadmini K, Smaoui A, Abdelly C, et al. Antimicrobial activities and phytochemical analysis of Tamarix gallica extracts. Ind Crops Prod. 2015; 76: 1114-22. DOI: 10.1016/j.indcrop.2015.08.020. [DOI:10.1016/j.indcrop.2015.08.020]
7. Lefahal M, Benahmed M, Louaar S, Zallagui A, Duddeck H, Akkal S. Antimicrobial activity of Tamarix gallica L. extracts and isolated flavonoids. Advances in Natural and Applied Sciences. 2010:289-93. URL: http://www.aensiweb.com/old/anas/2010/289-292.pdf
8. Cheng C, Min T, Luo Y, Zhang Y, Yue J. Electrospun polyvinyl alcohol/chitosan nanofibers incorporated with 1,8-cineole/cyclodextrin inclusion complexes: Characterization, release kinetics and application in strawberry preservation. Food Chem. 2023; 418: 135652. DOI: 10.1016/j.foodchem.2023.135652 [DOI:10.1016/j.foodchem.2023.135652] [PMID]
9. Liu Y, Wang D, Sun Z, Liu F, Du L, Wang D. Preparation and characterization of gelatin/chitosan/3-phenylacetic acid food-packaging nanofiber antibacterial films by electrospinning. Int J Biol Macromol. 2021;169:161-70. DOI: 10.1016/j.ijbiomac.2020.12.046 [DOI:10.1016/j.ijbiomac.2020.12.046] [PMID]
10. Venkatachalam K, Lekjing S. A chitosan-based edible film with clove essential oil and nisin for improving the quality and shelf life of pork patties in cold storage. RSC Adv. 2020;10(30):17777-86. DOI: 10.1039/d0ra02986f [DOI:10.1039/D0RA02986F] [PMID] [PMCID]
11. Han C, Wang J, Li Y, Lu F, Cui Y. Antimicrobial-coated polypropylene films with polyvinyl alcohol in packaging of fresh beef. Meat Sci. 2014 Feb;96(2 Pt A):901-7. DOI: 10.1016/j.meatsci.2013.09.003. [DOI:10.1016/j.meatsci.2013.09.003] [PMID]
12. Tripathi S, Mehrotra GK, Dutta PK. Physicochemical and bioactivity of cross-linked chitosan-PVA film for food packaging applications. Int J Biol Macromol. 2009;45(4):372-6. DOI: 10.1016/j.ijbiomac.2009.07.006 [DOI:10.1016/j.ijbiomac.2009.07.006] [PMID]
13. Nasiri A, Zeinali T, Ansarifar E. Electrospun Polyvinyl Alcohol/Chitosan Nanofibers Film Loaded With Artemisia Essential Oil: A Strategy for Extending Shelf Life and Enhancing Quality of Minced Beef. Food Sci Nutr. 2025 Dec 12;13(12):e71330. DOI: 10.1002/fsn3.71330. [DOI:10.1002/fsn3.71330] [PMID] [PMCID]
14. Pezeshk S, Rezaei M, Hosseini H. Antibacterial and antioxidant activities of shallot extract (Allium ascalonicum) on rainbow trout (Oncorhynchus mykiss) during chilled. Iranian J Nutr Sci Food Technol. 2011;6(2):0. [Persian]. URL: http://nsft.sbmu.ac.ir/article-1-503-en.html
15. Fatehi F, Khalili Sadrabad E, Feilizadeh M, Derakhshan Z, Heidari Kochaki S, Hekmatimoghaddam S, et al. Designing the pH-sensitive indicator based on starch nanoparticle with bromocresol green for monitoring meat spoilage. Int J Environ Health Res. 2025; 35(5):1204-12. DOI: 10.1080/09603123.2024.2383427 [DOI:10.1080/09603123.2024.2383427] [PMID]
16. Ahmadi A, Alishahi A. Investigating the substitution of sodium acetate with different chitosans in (Huso huso) fillet during storage in refrigerator (4±1 0C). Journal of Utilization and Cultivation of Aquatics. 2018;6(4):1-11. DOI: 10.22069/japu.2018.13433.1383
17. Shirvash M, Najafi A. Investigating the effect of nano-encapsulated peppermint essential oil on shelf life of rainbow trout fish burger during storage. Journal of food science and technology (Iran). 2024;21(152):94-106. [Persian]. DOI: 10.22034/FSCT.21.152.94
18. Pezeshky A, Mehrvand A, Khakbaz Heshmati M, Emamikhah F. The effect of the use of natural antioxidants (thyme and ginger oleoresin) on the physical, chemical and sensory properties of sesame oil. Food Research Journal, 2025; 35(4): 25-37. DOI: 10.22034/fr.2025.53919.1850
19. Shokri Tometri S, Ahmady M, Ariaii P, Soltani M. Effect of Nano-Liposome and Free Extract of Bay Leaf on The Shelf Life of Minced Beef during Refrigerated Storage. J Innov Food Sci Technol. 2022;14(54):33-49. DOI: 10.30495/jfst.2021.684156 URL: https://sanad.iau.ir/Journal/jfst/Article/1004632
20. Pilmal M, Alizadeh Doughikollaee E, Yousef Elahi M. Effect of Edible Chitosan Coating Containing Cinnamon Essential Oil on the Shelf Life of Silver Carp Fish Finger during Refrigerated Storage. Journal of Fisheries. 2018;71(3):294-305. DOI: 10.22059/jfisheries.2019.267297.1053 URL: https://jfisheries.ut.ac.ir/article_71767.html?lang=en
21. Bagheri R, Aryaee P. The Effect of Chitosan along with Basil Seed Gum and Nettle Essential Oil on the Microbial, Chemical, and Sensory Properties of Hamburger. Food Engineering Research. 2022;21(1):75-92. [Persian]. DOI: 10.22092/fooder.2022.357112.1322
22. Karimi M, Khodanazary A, Hosseini SM. The effect of film and coating of chitosan with vacuum packaging on quality characteristics of Spangled emperor (Lethrinus nebulousus) fillets stored at 4 ºC. Iranian Food Science and Technology Research Journal. 2019;15(5):597-611. [Persian] DOI: 10.22067/ifstrj.v15i4.76581
23. Valipour Kootenaie F, Ariaii P, Khademi Shurmasti D, Nemati M. Effect of chitosan edible coating enriched with eucalyptus essential oil and α‐tocopherol on silver carp fillets quality during refrigerated storage. Journal of food safety. 2017;37(1):e12295. DOI: 10.1111/jfs.12295 [DOI:10.1111/jfs.12295]
24. Alboghbeish H, Khodanazary A. Comparative effects of chitosan and nanochitosan coatings enriched with green tea)Camellia sinensis L.( extract on quality of Costal trevally fish (Carangoides coeruleopinnatus) during refrigerated storage. Iranian Scientific Fisheries Journal. 2018;26(5):95-109. DOI: 10.22092/isfj.2017.115005
25. Sallam KI. Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control. 2007;18(5):566-75. DOI: 10.1016/j.foodcont.2006.02.002 [DOI:10.1016/j.foodcont.2006.02.002] [PMID] [PMCID]
26. El-Shamery GE-DM. Studies on contamination and quality of fresh fish meats during storage. Egypt Acad J Biol Sci G Microbiol. 2010;2(2):65-74. DOI:10.21608/eajbsg.2010.16710 [DOI:10.21608/eajbsg.2010.16710]
27. Yaghoubi M, Ayaseh A, Alirezalu K, Nemati Z, Pateiro M, Lorenzo JM. Effect of chitosan coating incorporated with Artemisia fragrans essential oil on fresh chicken meat during refrigerated storage. Polymers. 2021;13(5):716. DOI: 10.3390/polym13050716 [DOI:10.3390/polym13050716] [PMID] [PMCID]
28. Naghdi S, Rezaei M, Bahramifar N. Evaluation Quality oxidation Minced of Common Kilka (Clupeonella cultriventris caspia) in Cold Storage Conditions by Sensor Bromophenol Blue Base on Chitosan Film. Journal of Fisheries Science and Technology. 2018;7(4):243-8. URL: https://jfst.modares.ac.ir/article_21300.html?lang=en
29. Khare AK, Abraham RJ, Rao VA, Babu RN, Ruban W. Effect of chitosan coating enriched with cinnamon oil (Cinnamomum zeylanicum) on storage stability of refrigerated chicken meat nuggets. Journal of Animal Research. 2016;6(2):181-94. DOI:10.5958/2277-940X.2015.00160.6 [DOI:10.5958/2277-940X.2015.00160.6]
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