Volume 30, Issue 4 (Januray 2024)
J Birjand Univ Med Sci. 2024, 30(4): 333-342 |
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Research code: 616/19
Ethics code: IR.BUMS.REC.1400.272
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Abbasnia V S, Eslimi Esfahani D, Khazdair M R, Oryan S, Foadoddini M. Investigating the relaxant effect of aqueous-alcoholic extract of Melissa Officinalis on smooth muscle of isolated rat trachea and its potential mechanisms. J Birjand Univ Med Sci. 2024; 30 (4) :333-342
URL: http://journal.bums.ac.ir/article-1-3369-en.html
URL: http://journal.bums.ac.ir/article-1-3369-en.html
Vahideh Sadat Abbasnia1 
, Delaram Eslimi Esfahani1 , Mohammad Reza Khazdair * 2, Shahrbanoo Oryan1 , Mohsen Foadoddini3
, Delaram Eslimi Esfahani1 , Mohammad Reza Khazdair * 2, Shahrbanoo Oryan1 , Mohsen Foadoddini3
1- Department of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2- Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran , m.khazdair@yahoo.com
3- Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
2- Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran , m.khazdair@yahoo.com
3- Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Abstract: (293 Views)
Background and Aims: Melissa officinalis (M. officinalis) is a well-known medicinal plant with various therapeutic properties such as, antioxidant and anti-inflammatory effects. Pharmacological effects of this plant is attributed with its main phenolic compounds, rosmarinic acid. This research aimed to study the relaxant effect of the aqueous-alcoholic extract of Melissa officinalis on the isolated tracheal smooth muscle (TSM) of rat.
Materials and Methods: The relaxant potential of cumulative concentrations of the aqueous-alcoholic extract of Melissa officinalis (50, 100, 200 µg/ml) was compared with normal saline (1 milliliter of normal saline) as a negative control and three cumulative concentrations of theophylline (0.8, 0.4, 0.2 mM) as a positive control on the TSM of isolated rat. The relaxant effect of M. officinalis extract was examined on non-incubated and incubated TSM with propranolol (1 μM), atropine (1 μM), and chlorpheniramine (1 μM) as different antagonists of beta-2 adrenergic (β2-Adrenergic), muscarinic and H1 histamine receptors respectively.
Results: The relaxant effect of M. officinalis extract with all theophylline concentrations on the isolated tracheal smooth muscle exhibited a significant increase compared to saline (P<0.001 in all instances) in non-incubated tissues. The relaxant effect of medium and high dose of M. officinalis extracts (100 and 200 µg/ml) were similar to theophylline and were not significant difference. Conversely, the relaxant effect of M. officinalis exhibited a significant reduction in incubated tissues with chlorpheniramine and atropine compared to the non-incubated tissue.
Conclusion: The relaxant effect of aqueous-alcoholic extract of Melissa officinalis on isolated tracheal tissue is possibly be mediated through the inhibition of muscarinic and H1 histamine receptors while having no significant impact on beta-2 adrenergic receptors.
Materials and Methods: The relaxant potential of cumulative concentrations of the aqueous-alcoholic extract of Melissa officinalis (50, 100, 200 µg/ml) was compared with normal saline (1 milliliter of normal saline) as a negative control and three cumulative concentrations of theophylline (0.8, 0.4, 0.2 mM) as a positive control on the TSM of isolated rat. The relaxant effect of M. officinalis extract was examined on non-incubated and incubated TSM with propranolol (1 μM), atropine (1 μM), and chlorpheniramine (1 μM) as different antagonists of beta-2 adrenergic (β2-Adrenergic), muscarinic and H1 histamine receptors respectively.
Results: The relaxant effect of M. officinalis extract with all theophylline concentrations on the isolated tracheal smooth muscle exhibited a significant increase compared to saline (P<0.001 in all instances) in non-incubated tissues. The relaxant effect of medium and high dose of M. officinalis extracts (100 and 200 µg/ml) were similar to theophylline and were not significant difference. Conversely, the relaxant effect of M. officinalis exhibited a significant reduction in incubated tissues with chlorpheniramine and atropine compared to the non-incubated tissue.
Conclusion: The relaxant effect of aqueous-alcoholic extract of Melissa officinalis on isolated tracheal tissue is possibly be mediated through the inhibition of muscarinic and H1 histamine receptors while having no significant impact on beta-2 adrenergic receptors.
*Corresponding Author: Mohammad Reza Khazdair; Emails: khazdairMR@Bums.ac.ir
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Type of Study: Original Article |
Subject:
Physiology
Received: 2023/12/27 | Accepted: 2024/01/31 | ePublished: 2024/02/4
Received: 2023/12/27 | Accepted: 2024/01/31 | ePublished: 2024/02/4
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