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Meshkini A, Rajabnia T. Reduction of cell growth and induction of apoptosis in osteosarcoma cells by silver nanoparticles. J Birjand Univ Med Sci.. 2017; 24
URL: http://journal.bums.ac.ir/article-1-2245-en.html

1- Assistant Professor Ferdowsi University of Mashhad , a.meshkini@um.ac.ir
2- BSC Ferdowsi University of Mashhad
Abstract:   (363 Views)

Background and Aim:  In this study, the synthesis of silver nanoparticle and the assessment of its toxicity against the proliferation of osteosarcoma cells were performed.

Materials and Methods: To confirm the production of silver nanoparticles, UV-Visible spectroscopy, X-ray diffraction and TEM analyses were performed. MTT assay was used to evaluate the toxicity of nanoparticles against cancer cells. The induction of apoptosis was indicated by acridine orange-ethidium bromide staining and flow cytometry (sub-G1).

Results:  Based on surface plasmon resonance, silver nanoparticles exhibit sharp band with maximum at 408 nm, indicating the synthesis of silver nanoparticles. TEM image showed that the nanoparticles have cubic shape with mean diameter of 65.5 nm. Further, XRD analyses revealed the synthesized nanoparticles have high crystallinity with 55 nm crystalite size. According to the MTT assay, nanoparticles have ability to decrease the viability of Saos-2 cells in a dose dependent manner. IC50 value corresponding to silver nanoparticles was estimated 21/1 µg/ml. Moreover, the effect of nanoparticles on tumor cells was accompanied by the cellular uptake of nanoparticle, cell cycle arrest at G2 phase and induction of cell death with mode of apoptosis.

Conclusion: Since silver nanoparticles have ability to reduce the proliferation of Saos-2 cells and to eradicate them by induction of apoptosis, these nanoparticles could be considered as a candidate for treating osteosarcoma cells.

Type of Study: Original Article | Subject: Biochemistry
Received: 2017/02/13 | Accepted: 2017/04/22 | Published: 2017/05/23

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