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Background and Aim: Pain management is very important in neonates. The use of non- pharmacological methods can be effective in reducing pain in neonates. The current study aimed at assessing the effect of non-nutritive sucking on the physiological and behavioral pain responses to B Hepatitis vaccine in term infants.

Materials and Methods: In this clinical- trial study in Ninth Day Hospital of Torbate Heidarryyeh in 2016, 60 qualified neonates randomly were assigned to two groups of experimental and control. In the experimental group, the neonates performed non-nutritive sucking through vaccination. In the control group, no particular interference was done. Data collection means included a demography questionnaire, a physiological responses record questionnaire, and the modified behavioral pain scale. Having collected the required data, analysis was performed through SPSS software (version 20).

Results: It was found that the mean of neonates’ behavioral responses scores was significantly different after the interference in the two groups (P<0.05). Before the interference, there was no significant difference in mean pulse rate between the groups (P>0.05) But, t-test results showed that after the interference, there was a significant difference in the two groups (P<0.05).Regarding the blood oxygen saturation, before the intervention, there was no significant difference between the two groups (P>0.05). But t-test results showed that after intervention it was significantly different in the two groups (P<0.05).

Conclusion: Non-nutritive sucking can be effective in the management of pain in neonates.