Volume 31, Issue 4 (February 2024)
J Birjand Univ Med Sci. 2024, 31(4): 311-320 |
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Research code: دانشگاه پیام نور با شماره 127/ع/64/1002
Ethics code: IR.PNU.REC.1402.156
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Nasri S, Shahi Sadrabadi F, Naseri M, Abbasi S. Effect of Melissa officinalis extract and Diazinon interaction on liver tissue and its enzymes alteration in male rats. J Birjand Univ Med Sci. 2024; 31 (4) :311-320
URL: http://journal.bums.ac.ir/article-1-3490-en.html
URL: http://journal.bums.ac.ir/article-1-3490-en.html
1- Department of Biology, Payame Noor University, Tehran, Iran , s_nasri2000@yahoo.com
2- Department of Biology, Payame Noor University, Tehran, Iran
3- Traditional Medicine Clinical Trial Research Center, Shahed University, Tehran, Iran
2- Department of Biology, Payame Noor University, Tehran, Iran
3- Traditional Medicine Clinical Trial Research Center, Shahed University, Tehran, Iran
Abstract: (461 Views)
Background and Aims: Diazinon is an organophosphorus poison and insecticide that has negative effects on the environment and human health. Lemon balm (Melissa officinalis) is one of the plants that is consumed and has antioxidant compounds. The aim of this study is to investigate the protective effect of lemon balm in diazinon treated rats.
Materials and Methods: The aerial parts extract of Melissa officinalis was prepared by maceration method. 56 male Wistar rats were divided to control, sham, diazinon-received(30mg/kg), lemon balm-received (50 and 100 mg/kg) and both diazinon and lemon balm-received groups. All injection was done intraperitoneally for 30 days. The level of alanine aminotransferase and alkaline phosphatase was measured by ELISA. After killing the animals, liver tissue was also stained with hematoxylin-eosin and observed by light microscope.
Results: Diazinon caused a significant increase of alkaline phosphatase and alanine aminotransferase and the number of Kupffer cells compared to the control group (P<0.05). In diazinon and Melissa officinalis extract receiving group, the level of these enzymes and Kupffer cells decreased compared to diazinon group(P<0.05). Diazinon decreased the number of hepatocytes compared to the control group(P<0.05). In diazinon and Melissa officinalis extract receiving group, the number of hepatocytes increased compared to the diazinon group(P<0.05).
Conclusion: Lemon balm extract improved the effects of diazinon on the liver of mice receiving diazinon.
Materials and Methods: The aerial parts extract of Melissa officinalis was prepared by maceration method. 56 male Wistar rats were divided to control, sham, diazinon-received(30mg/kg), lemon balm-received (50 and 100 mg/kg) and both diazinon and lemon balm-received groups. All injection was done intraperitoneally for 30 days. The level of alanine aminotransferase and alkaline phosphatase was measured by ELISA. After killing the animals, liver tissue was also stained with hematoxylin-eosin and observed by light microscope.
Results: Diazinon caused a significant increase of alkaline phosphatase and alanine aminotransferase and the number of Kupffer cells compared to the control group (P<0.05). In diazinon and Melissa officinalis extract receiving group, the level of these enzymes and Kupffer cells decreased compared to diazinon group(P<0.05). Diazinon decreased the number of hepatocytes compared to the control group(P<0.05). In diazinon and Melissa officinalis extract receiving group, the number of hepatocytes increased compared to the diazinon group(P<0.05).
Conclusion: Lemon balm extract improved the effects of diazinon on the liver of mice receiving diazinon.
Keywords: Lemon balm, Diazinon, Alkaline phosphatase, Alanine aminotransferase, Kupffer cells, Hepatocyte
Type of Study: Original Article |
Subject:
Pharmacology- Medicinal plants
Received: 2024/12/31 | Accepted: 2025/03/10 | ePublished: 2024/12/25
Received: 2024/12/31 | Accepted: 2025/03/10 | ePublished: 2024/12/25
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