Showing 4 results for Essential Oil
Hassan Ahmadvand, Hamzeh Amiri, Hamid Dalvand, Shahrokh Bagheri,
Volume 20, Issue 4 (12-2013)
Abstract
Background and Aim: Consumption of plant derived antioxidant compounds contributes to reducing risks of certain chronic and degenerative diseases. The present study aimed at comparing various antioxidative activities hydroalcholic of flaver extract (DKME) and essential oil of Artemisa persica in Lorestan province in 2012. Materials and Methods: The present experimental study was carried out in Lorestan province in 2012 . After supplying hydroalcholic extract and essential oil of Artemisa persica , radical scavenging activity of the samples was assessed by using diphenylpicrylhydrazyl (DPPH). Total antioxidant capacity of the samples was assessed through phosphomolybdat method. The amount of total phenol and flavonoid samples was assessed by means of Folin-Ciocalteu and Zhishen methods. The obtained data was analysed using Kruskal-Wallis test and SPSS software (V: 13) at the significant level (P<0.05). Results: It was found that total antioxidant capacity of hydroalcholic extract and essential oil of Artemisa persica was 25.123±5.18 and 1.05±0.55 nmol of ascorbic acid equivalent/g extract, respectively.That of essential oil and phenol content was 326.68±10.07 and 131.14±3mg of gallic acid equivalent (GAE)/g extract or essential oil, respectively .Flavonoid content of quercetin equivalents/g extract and essential oil was 6.3±0.94 and 1.4±0.65 mg , in the order mentioned. In the DPPH scavenging assay, the IC50 (the concentration required to scavenge 50% of radicals) values of hydroalcholic extract of Artemisa Persica leaves, essential oil and Butylated hydroxytoluene (BHT) as reference was 165.73±4, 16470.67±34, and 3.88±1μg/ml, respectively. Conclusion: The current study showed that Artemisa persica extract is an easily accessible source of natural antioxidants which can be suitable to be included in foods and pharmaceutical applications.
Majid Zare Bidaki, Mina Arab, Mohtarame Khazaei, Ehsan Afkar,
Volume 21, Issue 3 (12-2014)
Abstract
Background and Aim: Nowadays resistance to antibiotics and their side effects has emerged as a worldwide problem. As a result, tend to use anti-bacterial compounds of plant origin has been increased. Mint plant scientifically called Mentha spicata L. is one of the plants which has many medicinal uses and its antibacterial effects is a matter of debate. We aimed to study antibacterial effects of Mentha spicata L essential oil on 8 standard bacterial species including Escherichia coli, Bacillus cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Shigella flexneri, and two Salmonella species. Materials and Methods: In this experimental study, using micro-dilution broth method and reading the results by ELISA reader, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of mint essential oil were determined against the 8 standard bacterial strains. The lab tests were repeated for three times. The results were analyzed by statistical tests. Results: The MIC for Mentha spicata L essential oil was significantly different among the bacterial species (p <0.05). The highest growth inhibitory effect was found against Bacillus cereus and the least ones were recorded against Klebsiella pneumoniae and Staphylococcus aureus. No significant difference was found between the bacterial MBCs (p> 0.05). Conclusion: This study revealed significant and selective antibacterial characteristics for Mentha spicata L suggesting to be used in pharmaceutical and food industries.
Arezou Alipour Kakroudi, Somayeh Rahaiee, Hajar Rajaei Litkohi, Saeed Ghanbari Hassan Kiadeh,
Volume 28, Issue 4 (12-2021)
Abstract
Background and Aims: Herbal essential oils (EOs) have antimicrobial and antioxidant activities due to the high amount of bioactive compounds; therefore, they are considered good candidates for applications in the food and pharmaceutical industries. The present study aimed to assess the total phenolic content and in vitro comparative study of the biological activities of EOs from different plants (e.g., clove, common sage, savory, and organum).
Materials and Methods: In this experimental study, total phenolic content in EOs was determined, and their antioxidant capacity was measured by the DPPH free radical scavenging method. The components of essential oil were identified using a Gas chromatography-mass spectrometry (GC-MS) device. Moreover, the antibacterial activity of EOs was evaluated by the disk diffusion method, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated by the broth macro dilution method. Data were analyzed using one-way analysis of variance (ANOVA) and Duncan's multiple range test.
Results: The highest content of total phenol (157.07±2.37 mg GAL/g dry weight) was recorded for EO of organum. Moreover, the highest percentage of free radical scavenging was determined at 98.142% for organum. The results of GC/MS analysis depicted that monoterpenes were the main compounds of Eos of organum, and the highest value was obtained for the alpha-pinene (74.04%). Furthermore, the results of antibacterial activity of EOs demonstrated that the highest zone of inhibition with a diameter of 44±0.81 mm was observed for the EO of organum. The lowest values of MIC and MBC were reported as 0.275 and 0.55 mg/mL for the EOs of organum and savory against gram-positive Bacillus cereus, respectively.
Conclusion: As evidenced by the results of the current study, the assessed Eos, specifically those of organum, have effective antioxidant and antibacterial activity against bacteria strains, especially gram-positive ones, and can be used as safe antimicrobial compounds in food and health products.
Gholrokh Farokhmehr, Ghader Najafi, Saeid Abbasi Maleki,
Volume 29, Issue 4 (12-2022)
Abstract
Background and Aims: The opioid system plays a key role in opioid tolerance and dependence. Furthermore, studies have shown that the opioid system plays a role in the analgesic effects of Ferula gummosa extract. Therefore, the present study aimed to investigate the effect of Ferula gummosa essential oil (FGEO) on morphine tolerance and dependence in mice.
Materials and Methods: In this experimental study, 60 albino mice were divided into 10 groups of 6 as follows: carrier (10 ml/kg), diazepam (5 mg/kg), and FGEO (40, 20, and 10 mg/kg). To induce morphine tolerance or dependence, it was administrated 3 times for 3 days. In the tolerance phase, drugs were injected intraperitoneally for 30 minutes before morphine administration. In the dependence phase, the drugs were used only on the 4th day (test day). The withdrawal signs were recorded for 30-min. The data were analyzed in SPSS software (version 20) through ANOVA and Kruskal-Wallis statistical tests, followed by Tukey's and Ben Feroni's post hoc tests..
Results: In total, 20 and 40 mg/kg doses of FGEO in the tolerance phase and only the high dose of the essential oil (40 mg/kg) decreased the number of jumps in the dependence phase. Other behaviors (e.g., diarrhea, stomach cramps, standing on two feet, climbing, wet dog shakes, self-treatment, and teeth chattering) decreased by FGEO in both phases.
Conclusion: FGEO decreased morphine tolerance and dependence and possibly was useful for the treatment of opioid dependence after complimentary trials.
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