per
Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
1
9
article
The effect of non-nutritive sucking on Physiological and Behavioral Pain Responses Caused by First Turn Hepatitis B Vaccine in Term Infants
Moosa Sajjadi
sajjadi1975@gmail.com
1
Mahdi Basirimoghaddam
basiri1344@gmail.com
2
Maryam Tatari
maryamtatary@yahoo.com
3
Elahe Amiri Shadmehri
amiri602013@gmail.com
4
Social Development & Health Promotion Research Center, Gonabad University of Medical Sciences, Gonabad, Iran.
Department of Pediatric Nursing, Faculty of Nursing & Midwifery, Social Development and Health Promotion Research Center, Gonabad University of Medical Sciences, Gonabad, Iran.
MSc of Biostatistics, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
MS in Nursing Education, Pediatric Subdisciplin, Faculty of Nursing & Midwifery, Gonabad University of Medical Sciences, Gonabad, Iran.
Background and Aim: Pain management is very important in neonates. The use of non- pharmacological methods can be effective in reducing pain in neonates. The current study aimed at assessing the effect of non-nutritive sucking on the physiological and behavioral pain responses to B Hepatitis vaccine in term infants.
Materials and Methods: In this clinical- trial study in Ninth Day Hospital of Torbate Heidarryyeh in 2016, 60 qualified neonates randomly were assigned to two groups of experimental and control. In the experimental group, the neonates performed non-nutritive sucking through vaccination. In the control group, no particular interference was done. Data collection means included a demography questionnaire, a physiological responses record questionnaire, and the modified behavioral pain scale. Having collected the required data, analysis was performed through SPSS software (version 20).
Results: It was found that the mean of neonates’ behavioral responses scores was significantly different after the interference in the two groups (P<0.05). Before the interference, there was no significant difference in mean pulse rate between the groups (P>0.05) But, t-test results showed that after the interference, there was a significant difference in the two groups (P<0.05).Regarding the blood oxygen saturation, before the intervention, there was no significant difference between the two groups (P>0.05). But t-test results showed that after intervention it was significantly different in the two groups (P<0.05).
Conclusion: Non-nutritive sucking can be effective in the management of pain in neonates.
http://journal.bums.ac.ir/article-1-2183-en.html
Non Nutritive Sucking
Physiological Responses
Behavioral Responses
Pain
Term Infants.
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Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
10
18
article
Comparison of AGTR1 rs5186 (A1166C) Polymorphism between Coronary Artery Disease Patients and Normal Subjects: a Case Control Study
Mohammad Mehdi Heidari
heidarimm@yazd.ac.ir
1
Shirin Ghasemi
Shiringa_13@gmail.com
2
Faeghe Haji hosseini
Hajihosseini_F@yahoo.com
3
Mehdi Hadadzadeh
Hadad_Mehdi@gmail.com
4
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
Department of Cardiac Surgery, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Background and Aim: Coronary artery disease (CAD) is a multifactorial inherited disorder in which the arteries that blood to the heart muscle become hardened and narrowed. We aimed at investigating the role of rs5186 (A1166C) polymorphism the angiotensin II type 1 receptor (AGTR1) gene as risk factors in some Iranian CAD patients.
Materials and Methods: In this case-control study 137 samples were selected from 141 CAD Iranian patients, who had had CABG surgery. Besides, 137 healthy controls matched for age, sex, and ethnicity were taken. After extracting DNA of the blood samples, A1166C AGTR1 gene polymorphism was studied using tetra-primer ARMS-PCR method. The results of a single tube T-ARMS-PCR were validated using DNA sequencing method. For genetic analysis dominant, co-dominant, and recessive models of multiple logistic regression were applied using SPSS software (V: 22).
Results: It was found that 67.4% of the cases belonged to AA genotype, genotypes amounted 26.9% to AC, and 5.7% to CC. A significant association of AGTR1 polymorphisms with CAD was found.
Conclusion: Polymorphism A1166C AGTR1 gene can have an effective role in increasing the risk of coronary artery disease; thus, it can be used in clinical studies.
http://journal.bums.ac.ir/article-1-2163-en.html
Key Words: CAD
Gene polymorphism
rs5186
AGRT1
T-ARMS-PCR.
per
Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
19
27
article
Preparation of tacrolimus ophthalmic solution using cyclodextrins
Farzaneh Hajiahmadi
hajiahmadi.farzaneh@yahoo.com
1
Akbar Derkhshan
derakhshana@mums.ac.ir
2
Maliheh Nikandish
nikandishm1@mums.ac.ir
3
Bizhan Malaekeh-Nikouei
malaekehb@mums.ac.ir
4
Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Ophthalmology, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Background and Aim: Application of topical tacrolimus in Vernal Keratoconjunctivitis is considered as an alternative to steroids. Due to the low aqueous solubility of the drug, preparing a solution of the drug has got particular importance. In the present study, tacrolimus eye drop was prepared using cyclodextrin, as a drug solubility enhancer.
Materials and Methods: To improve the aqueous solubility of tacrolimus, 5 types of cyclodextrins called α, γ, hydroxypropyl (HP)-β, HP-γ, and sulfobutyl ether (SBE)-β-cyclodextrin (CD), were used. After phase solubility studies, the most appropriate cyclodextrin was selected to prepare tacrolimus eye drop. For eye drop preparation, phosphate buffer (pH=7.4), hydroxypropyl methylcellulose (HPMC) and polyvinyl alcohol (PVA) polymers (viscosity increasing agent), methyl paraben and benzalkonium chloride (preservative agent) were used together with cyclodextrin-tacrolimus complex.
Results: α, HP-β, and γ-CD had the most effect on aqueous solubility, respectively. These 3 types of CDs, respectively increased the intrinsic solubility of tacrolimus174, 160, and 102 times. Taking this into account, α and HP-β-CD were chosen to prepare a soluble form of tacrolimus. In the eye drop formulation containing CD-tacrolimus complex, methyl paraben and HPMC, remarkably reduced the drug solubility while there was not a critical change in the solubility of tacrolimus in the case of CD-tacrolimus complex, buffer, HPMC or PVA and benzalkonium chloride.
Conclusion: It is possible to prepare tacrolimus eye drop (0.05%) using cyclodextrins.
http://journal.bums.ac.ir/article-1-2230-en.html
Tacrolimus
Cyclodextrin
Aqueous solubility
Eye drop.
per
Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
28
35
article
Vitamin D status among multiple sclerosis patients in South Khorasan: a case-control study
Masoumeh Zarezadeh
Masu.zare49@gmail.com
1
Mohammad Dehghani Firoozabadi
mdehghani44@yahoo.com
2
Asghar Zarban
azarban@yahoo.com
3
Gholamreza Sharifzadeh
rezamood@yahoo.com
4
Maryam Valavi
m_valavi68@yahoo.com
5
Member of Student Research Committee, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Department of Neurology, Faculty of Medicine, Birjand University of medical science, Birjand, Iran.
Birjand CardioVascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Social Determinant of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Member of Student Research Committee, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Background and Aim: Observational studies have demonstrated that the risk of developing multiple sclerosis (MS) is associated with vitamin D (25(OH)D) status. However, there is no local study regarding vitamin D status in MS patients in the South Khorasan. Therefore, present study aimed at comparing the serum levels of vitamin D between the South Khorasan MS patients and healthy subjects, and determining its association with disability in these patients.
Materials and Methods: In this case-control study a total of 144 individuals (72 previously diagnosed MS cases and 72 age and sex matched healthy controls) were included. Demographic characteristics and serum vitamin D levels of both groups as well as Expanded Disability Status Scale (EDSS) for MS patients were assessed. The obtained data was analyzed using t-test, Mann Whitney U test, Chi-square, and Pearson's correlation coefficient test applying SPSS software.
Results: There was a significant difference (P<0.0001) in vitamin D levels between the case (47.4±5.4ng/mL) and the control (14.66±10.4 ng/mL) groups. A direct association was found between vitamin D levels and EDSS in MS patients (P<0.0001), too.
Conclusion: Surprisingly, it was found that vitamin D level was significantly higher in MS patients than the controls. There was a significant positive association between the level of vitamin D and disability status in patients with MS. The association may be due to more administration of vitamin D supplement to MS patients.
http://journal.bums.ac.ir/article-1-2158-en.html
Multiple Sclerosis
EDSS
Vitamin D.
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Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
36
49
article
The efficiency of biosynthesis silica nanoparticles at removal of heavy metals Cr and Cu from aqueous solutions
Mohammad Hossein Sayadi
mh_sayadi@yahoo.com
1
Masoume Siami
masoomsiyami@gmail.com
2
Mohsen Esmailpour
hmsayadi@gmail.com
3
Mahmud Hajiani
hajiani59@gmail.com
4
Department of Environment, School of Natural Resources and Environment, University of Birjand, Birjand, Iran.
Department of Environment, School of Natural Resources and Environment, University of Birjand, Birjand, Iran.
Department of Chemistry, School of Science, University of Shiraz, Shiraz, Iran.
Department of Environment, School of Natural Resources and Environment, University of Birjand, Birjand, Iran.
Background and Aim: Nanotechnology considered as one of the main management strategy in reducing negative effects of water pollution, which leads to treating of water inexpensive and more effective. This study aimed to investigate the removal of Cr and Cu from aqueous solutions using nano-silicate which is synthesized by rice husk.
Materials and Methods: In the present study, functionalized nano-silica with rice husk was fabricated and characterized by FT-IR، TEM, SEM and XRD. The effect of adsorbent dosage, initial concentration and equilibrium of chromium and copper and contact time in removal of mentioned heavy metals were investigated. Finally, the adsorption isotherms were analyzed.
Results: The silica nanoparticles had nearly spherical morphology with a uniform size of about 80 nm. However, the obtained functionalized silica nanoparticles were spherical (about 90 nm in diameter). Results showed that optimum adsorption of Cr and Cu contaminant obtained at 100 minutes, while the optimum amount of adsorbent for Cr and Cu were 125 and 100 mg respectively. The optimal concentration of Cr and Cu was 2 mg/l. The correlation coefficients of adsorption isotherms of Cr (R2Langmuir =0.9946) and Cu (R2Langmuir =0.999) showed the good agreement between the adsorption data and Langmuir model.
Conclusion: The study showed that the silica nanoparticles can be produced using agricultural waste as a inexpensive and environmentally friendly method and can be used to removing of contaminants from the aquatic environment.
http://journal.bums.ac.ir/article-1-2180-en.html
Nano-silicate
Heavy metals
Adsorption isotherms
Rice husk.
per
Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
50
61
article
Study on effect of Artemisia sieberi hydro-alcoholic extract on the survival of Lactobacillus acidophilus and Bifidobacterium lactis in probiotic yoghurt
Saeed Akbari
saeed.akbari_1001@yahoo.com
1
Ataollah Azhdari
Ataazhdari@yahoo.com
2
Gholam Reza Sharifzadeh
rezamood@yahoo.com
3
Department of Food Science and Technology, faculty of Agriculture, Birjand Branch, Islamic Azad University, Birjand, Iran.
Department of Food Science and Technology, faculty of Agriculture, Birjand Branch, Islamic Azad University, Birjand, Iran.
Social Determinant of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Background and Aim: In the present study, the possibility of probiotic yoghurt production using Artemisia sieberi hydro- alcoholic extract and also the effects of different concentrations of this medicinal herb on the survival of two probiotic strains, Lactobacillus acidophilus and Bifidobacterium lactis, in probiotic yoghurt were investigated.
Materials and Methods: In different treatments, the amounts of 0, 0.1, 0.2, 0.3 and 0.4 gr/lit of Artemisia sieberi extract together with conventional yoghurt starter, Bif. lactis and lact. acidophilus were added to 1 liter of boiled milk. The samples were incubated at 37˚centigrade, and then, the acidity and pH changes every two hours during the incubation period were examined up to approximately 80˚ of the survival of probiotic bacteria was tested during the storage of the samples in the refrigerator. On the tenth day, after yoghurt production, all the samples were examined for sensory evaluation using a panel test and the obtained data was analyzed by means of SPSS software (V:19).
Results: There was no significant difference in the acidity and pH changes during the production process of probiotic yoghurt in different treatments. The probiotic yoghurt containing 0.4 gr/lit of Artemisia hydro-alcoholic extract had the best quality in terms of organoleptic properties and shelf life of the product. During 21 days storage in the refrigerator none of the treatments showed the number of probiotic bacteria less than 106 bacteria in gram.
Conclusion: It was found that appropriate concentrations of Artemisia sieberi extract can be used for the production of probiotic yoghurt, as a new functional food containing Lactobacillus acidophilus and Bifodobacterium lactis.
http://journal.bums.ac.ir/article-1-2132-en.html
Artemisia sieberi
Probiotic yoghurt
Lactobacillus acidophlus
Bifidobacterium lactis.
per
Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
63
72
article
The role of non- alcoholic fatty liver disease (NAFLD)in the occurrence of CVDs through measuring carotid intima-media thickness
Tahereh Fakharian
t.fakharian@gmail.com
1
Shima Heydari
shima.heydari_b83@yahoo.com
2
Ghodsiyeh Azarkar
gh.azarkar@yahoo.com
3
Ali reza Ehsan bakhsh
a.r.ehsanbakhsh@gmail.com
4
Gastrointrologist, Birjand University of Medical Sciences, Birjand, Iran.
Member of Student Research Committee, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Department of Radilogy, Birjand University of Medical Sciences, Birjand, Iran.
Department of Radilogy, Birjand University of Medical Sciences, Birjand, Iran.
Background and Aim: A lot of studies is done about various components of the metabolic syndrome in association with non-alcoholic fatty liver due to increasing carotid intima media thickness as a risk factor of cardiovascular diseases. But few studies have been done on the role of non-alcoholic fatty liver as an independent risk factor for carotid intima media thickening. The present study aimed at evaluating the effect of non-alcoholic fatty liver on the extent of carotid intima media thickness, as a risk factor for atherosclerosis.
Materials and Methods: In the current study, mean thickness of carotid intima media was measured in 105 patients divided into three equal groups. Two groups included patients with mild to severe fatty liver with or without cardiovascular risk factors, respectively. The third group was the control one. The obtained data was fed into SPSS software (V:22) using ANOVA and Tukey test at α=0.05.
Results: It was found that the thickness of the right carotid intima media in the control group was 0.5±0.09, but in the fatty liver groups ,with or without a risk factor, it was 0.26±0.08; respectively. But, in the left carotid intima media in the control groups having the same condition it was 0.8±0.18 and 0.82 ±0.26, respectively.
P value revealed that mean thickness of the right carotid intima media and the left one in the control groups was significantly less than that of fatty liver groups with or without risk factor.
Conclusion: Fatty liver disease leads to atherosclerotic arteries and thickening of the carotid artery as an indicator of atherosclerotic intima media, which is even visible when a mild degree of fatty liver is concerned.
http://journal.bums.ac.ir/article-1-2095-en.html
Non-alcoholic fatty liver disease
Carotid artery
Atherosclerotic carotid intima media thickness.
per
Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
73
78
article
Prevalence of microbial contamination of traditional ice-creams in ice-cream supplier trade units in Birjand in 2015: Short Communication
Mohadeseh Abolhasannezhad
rezamood@yahoo.com
1
Gholamreza Sharifzadeh
atefe_nakhaei@bums.ac.ir
2
Kobra Naseri
naserik@bums.ac.ir
3
Azarmdokht Abedi
azarmabedi@yahoo.com
4
Saeed Yosefi
m_abolhasannezhad@yahoo.com
5
Atefeh Nakhaei
chemist1407@gmail.com
6
MSc of Food Science and Industry, Metabolism and Antioxidants Research Center, Birjand University of Medical Sciences, Birjand, Iran
Social Determinant of Health Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Department of Pharmacology, Birjand University of Medical Sciences, Birjand, Iran.
Employee, Food and Drugs Laboratory, Birjand University of Medical Sciences, Birjand, Iran.
Employee, Food and Drugs Laboratory, Birjand University of Medical Sciences, Birjand, Iran.
Employee, Food and Drugs Laboratory, Birjand University of Medical Sciences, Birjand, Iran.
Traditional ice-cream is a type of ice-cream whose all processes of production and distribution are done by hand. The present study aimed at assessing the prevalence of bacterial contamination of traditional ice creams and its decisive factors.
The current study was a descriptive cross-sectional one in Birjand in 2015. The samples were transferred under sterile conditions and cold chain, to a food laboratory. They went through laboratory tests of Enterobacteriaceae bacteria, Escherichia coli, and Staphylococcus aureus Cocos. The obtained results were analyzed using SPSS statistical software ( V:15).
The results showed that from among 96 samples collected 25 samples were acceptable while 71 samples were unusable. Maximum contaminations were due to Enterobacteriaceae in 39 cases (40%), and Staphylococcus aureus infection was found in 27 cases, (28%) the least contamination-14 (15%) cases -belonged to E. coli. In addition 39 infection (42.4%) 8 (8.69%) was because of coliforms. Besides, 8 cases were simultaneously contaminated with coliforms, escherichia coli and staphylococcus aureus, 12 samples (13.04%) with coliforms, Escherichia coli, and Staphylococcus aureus; and 2 samples (2.17%) were simultaneously contaminated with coliforms and E. coli.
Birjand traditional ice-creams are highly contaminated with bacteria, which is a sign of poor hygiene in the preparation and distribution of this product.
http://journal.bums.ac.ir/article-1-2160-en.html
Traditional Ice Cream
Infectious Pathogens
Microbial Contamination
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Birjand University of Medical Sciences
Journal of Birjand University of Medical Sciences
1607-2197
2423-6152
2017-05
24
1
79
83
article
Comparison of Molecular methods in the Identification of Mycobacterium tuberculosis strains
Masoud Keikha
masoud.keykha90@gmail.com
1
Department of Medical Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Tuberculosis is one of the priorities of the World Health Organization of which millions of people in the world die annually. For the control and eradication of the disease, in addition of strategies like treatment of patients and identification of those with drug-resistant TB, We also need to detect the source of infection and track the disease’s transmission manner. Molecular epidemiology is one of the most important means to achieve this goal. The present study aimed at declaring the need of molecular identification and differentiation between strains of Mycobacterium tuberculosis, as one of the TB control strategies.
http://journal.bums.ac.ir/article-1-2241-en.html
Tuberculosis
Molecular Epidemiology
Tuberculosis transmission.