fa بررسی اثر نانوذرات سیلیکا آمینه بارگذاری شده با کورکومین در تغییر الگوی بیان ژن‌های آپوپتوزی در رده سلولی RPMI-8226 مالتیپل میلوما هماتولوژي Hematology مقاله اصیل پژوهشی Original Article <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:">زمینه و هدف:</span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">مالتیپل میلوما یک بدخیمی خونی غیرقابل درمان پلاسماسل</span></span></span></span>&nbsp;<span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">است که در آن اختلال تنظیم فرایند آپوپتوز رخ می‌دهد. هدف از این مطالعه، بررسی اثر نانوذرات سیلیکای مزومتخلخل عامل‌دار شده با آمین بارگذاری شده با کورکومین (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MSNs-NH₂-Cur</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">) بر الگوی بیان‌ژن‌های محوری مرتبط با آپوپتوز در رده سلولی مالتیپل میلوما </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">RPMI-8226</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> بود.</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:">روش تحقیق: </span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:">در این مطالعه تجر<u>بی</u>، سلول‌های </span></span></span><span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">RPMI-8226</span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">با </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MSNs-NH₂-cur</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> در غلظت</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> IC₅₀ </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">به مدت 24 ساعت تیمار شدند</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">کارایی</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> PCR </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">با</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> LinRegPCR </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">محاسبه</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">شد و </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">سطوح نسبی بیان ژن‌های</span></span></span></span> <i><span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P53</span></span></span></span></i><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">، </span></span></span></span><i><span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bax</span></span></span></span></i><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">، </span></span></span></span><i><span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bcl-2</span></span></span></span></i><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> و </span></span></span></span><i><span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Beclin-1</span></span></span></span></i> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">به روش</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> qRT-PCR </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">اندازه‌گیری و تحلیل شد</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:">یافته‌ها:</span></span></span></b> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MSNs-NH₂-Cur</span></span></span></span> &nbsp;<span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">باعث افزایش معنی‌دار بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">TP53</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> در سطح رونویسی شد </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(P<0.01)</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">.</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">در مقابل، تغییر معناداری در بیان </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Beclin-1</span></span></span></span><b> </b>&nbsp;<span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">و نسبت </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bax/Bcl-2</span></span></span></span><b> </b>&nbsp;<span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">مشاهده نگردید (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P>0.05</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">). این نتایج نشان‌دهنده</span></span><span new="" roman="" style="font-family:" times=""><span style="color:black">‌</span></span><span b="" mitra="" style="font-family:"><span style="color:black">ی فعال‌سازی پاسخ وابسته به</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> p53 </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">در این مدل سلولی است</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span><b><u> </u></b><b><u><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></u></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">نتیجه‌گیری:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">این مطالعه نشان داد </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MSNs-NH₂-Cur</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">موجب افزایش معنی‌دار بیان ژن </span></span></span></span><span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">TP53</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"> شد، </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">در حالی‌که تغییر قابل‌توجهی در شاخص‌های میتوکندریایی و اتوفاژی مشاهده نشد. این یافته‌ها نشان‌دهنده فعال‌سازی محور</span></span></span></span> <span dir="LTR" lang="EN-US" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p53</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black">بوده و نیازمند تأیید در سطح پروتئین و انجام مطالعات تکمیلی است</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:10.0pt"><span style="line-height:107%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:107%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Background and Aims:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> <span style="color:black">Multiple myeloma is an incurable plasma cell hematologic malignancy characterized by dysregulation of the apoptotic process. The present study aimed to evaluate the effect of curcumin-loaded amine-functionalized mesoporous silica nanoparticles (MSNs-NH₂-Cur) on the expression pattern of key apoptosis-related genes in the RPMI-8226 multiple myeloma cell line.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> <span style="color:black">In this experimental study, RPMI-8226 cells were treated for 24 hours with MSNs-NH₂-Cur at the previously determined IC₅₀ concentration. PCR efficiency was calculated using LinRegPCR software, and the relative mRNA expression levels of <i>P53</i>, <i>Bax</i>, <i>Bcl-2</i>, and <i>Beclin-1</i> were quantified and analyzed by quantitative real-time PCR (qRT-PCR).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> <span style="color:black">MSNs-NH₂-Cur treatment resulted in a significant upregulation of <i>TP53</i> at the transcriptional level (P < 0.01). In contrast, no significant changes were observed in <i>Beclin-1</i> expression or in the <i>Bax/Bcl-2</i> ratio (P > 0.05). These findings suggest activation of a p53-dependent response in this cellular model.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="font-family:Calibri,sans-serif"><b><span lang="EN-US" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The study demonstrated that MSNs-NH₂-Cur significantly upregulated <i>TP53</i> gene expression, whereas no notable changes were observed in mitochondrial or autophagy-related markers. These findings indicate activation of the p53 pathway and warrant further validation at the protein level and additional follow-up studies.</span></span></span></span></span><br> <br> &nbsp;  آپوپتوز, p53, نانوذرات سیلیکا آمینه بارگذاری شده با کورکومین, مالتیپل میلوما Apoptosis, p53, Curcumin-loaded amine-functionalized mesoporous silica nanoparticles, Multiple myeloma 0 0 http://journal.bums.ac.ir/browse.php?a_code=A-10-785-8&slc_lang=fa&sid=1 Maziar Nasiri مازیار نصیری maziar.nasiri@bums.ac.ir 8700319475328460051894 8700319475328460051894 No Mahtab Sayadi مهتاب صیادی Sayadi.mahtab@yahoo.com 8700319475328460051895 8700319475328460051895 No Mehdi Shakibaie مهدی شکیبایی shakibaie_m@yahoo.com 8700319475328460051896 8700319475328460051896 No Elias Sadooghi Rad الیاس صدوقی راد elyas1377@gmail.com 8700319475328460051897 8700319475328460051897 No Gholamreza Anani Sarab غلامرضا عنانی سراب ghansa@yahoo.com 8700319475328460051898 8700319475328460051898 Yes