fa اثرات لیراگلوتاید روی شاخص‌های بیوشیمیایی پلاسما و بافت بیضه با القاء دیابت و چاقی در موش فيزيولوژي Physiology مقاله اصیل پژوهشی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">زمینه و هدف:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">چاقی <span style="color:black">و</span></span></span> <span style="font-size:9.0pt"><span style="color:black"><a href="https://www.sid.ir/search/paper/%D8%AF%DB%8C%D8%A7%D8%A8%D8%AA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" style="color:blue; text-decoration:underline" target="_blank"><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">دیابت</span></span></span></a></span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">از</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> شایع‌ترین اختلالات متابولیکی هستند</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> هردو اثرات منفی بر سیستم بدن از جمله دستگاه تولیدمثلی می‌گذارند، از طرفی درمان داروئی آن‌ها باعث اختلال در باروری می&shy;شوند. از جمله داروهای کاندید برای درمان این بیماری‌ها لیراگلوتاید است. پژوهش حاضر با هدف بررسی تأثیر داروی لیراگلوتاید روی بافت بیضه و فراسنجه&shy;های خونی به‌ویژه پروتئین واکنشی</span></span><span dir="LTR" style="font-size:9.0pt">C </span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">&nbsp;(شاخص التهاب) انجام شد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">روش تحقیق:</span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> <span style="color:black">در این مطالعه تجربی </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">تعداد 40 سر موش نر سوری به 5 گروه با 8 تکرار تقسیم شدند که شامل گروه&shy;های شاهد، چاق، گروه چاق دریافت کننده لیراگلوتاید </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="color:black">mg/kg</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">2/1، گروه دیابتی و گروه دیابتی دریافت کننده لیراگلوتاید با دوز 2/1 میلی&shy;گرم بر کیلوگرم وزن بدن بودند. لیراگلوتاید به‌صورت روزانه و زیر جلدی تزریق گردید. پس از شش هفته مطالعه حیوانات بیهوش و نمونه</span></span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">سرم تهیه و مقاطع بافت بیضه </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">تهیه شدند. </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">مقاطع بافتی براساس روش&shy;های رایج تهیه گردید.</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">یافته‌ها:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">لیراگلوتاید قطر لوله&shy;های اسپرم‌ساز را در گروه&shy;های چاق و دیابتی افزایش داد</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black"> (</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">01/0></span></span></span><span dir="LTR" style="color:black">P</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">. دیابت و چاقی ضخامت لایه اپیتلیوم لوله&shy;های اسپرم‌ساز را کاهش دادند </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">(</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">01/0></span></span></span><span dir="LTR" style="color:black">P</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">. پروتئین&shy; واکنشی </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="color:black">C</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black"> در گروه&shy;های دیابتی و چاق که به آن‌ها لیراگلوتاید تزریق شد کاهش یافت</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black"> (</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">01/0></span></span></span><span dir="LTR" style="color:black">P</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">.</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">نتیجه‌گیری:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">لیراگلوتاید می‌تواند سبب بهبود سیستم تولید‌مثلی جنس نر (بافت بیضه) و کاهش التهابات مزمن در زمان چاقی و دیابت شود.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims:</span></b> <span lang="EN" style="font-size:11.0pt">Obesity and diabetes are the most common metabolic disorders that have negative effects on organisms, including the reproductive system. The drug treatments cause fertility disorders. One of the candidate drugs is liraglutide. The present study sought to assess the effect of liraglutide on testicular tissue and blood parameters, especially C-reactive protein (inflammation index).</span><span style="font-size:11.0pt"></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods:</span></b><span style="font-size:11.0pt"> A total of </span><span lang="EN" style="font-size:11.0pt">40 adult male mice were assigned to five groups with eight replications. These groups included control, obese, obese+liraglutide injected 1.2 mg/kg of body weight, diabetes, and the last group, diabetes+liraglutide injected 1.2 mg/kg of body weight. Liraglutide was injected subcutaneously daily. The duration of the experiment was six weeks. After the end of the experiment, the animals were anesthetized, and blood samples</span> <span lang="EN" style="font-size:11.0pt">a</span><span style="font-size:11.0pt">nd testicular sections</span><span lang="EN" style="font-size:11.0pt"> were taken. Animals were euthanized (cervical dislocation). The testicles were removed and fixed. The tissue sections were prepared according to the usual protocol.</span><span style="font-size:11.0pt"></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b> <span lang="EN" style="font-size:11.0pt">The results demonstrated that the diameter of</span> <span style="font-size:11.0pt">seminiferous tubules</span><span lang="EN" style="font-size:11.0pt"> in obese and diabetic groups was increased by liraglutide injection (P<0.01). Diabetes and obesity decreased the thickness of the germ cell epithelium of</span> <span style="font-size:11.0pt">seminiferous tubules </span><span lang="EN" style="font-size:11.0pt">(P<0.01). The C-reactive protein decreased in diabetic and obese groups when injected</span><span style="font-size:11.0pt"> with </span><span lang="EN" style="font-size:11.0pt">liraglutide (P<0.01).</span><span style="font-size:11.0pt"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion:</span></b><span style="font-size:11.0pt"> <span style="color:black">As evidenced by the results obtained, liraglutide can improve the male reproductive system and reduce chronic inflammation caused by</span></span><span lang="EN" style="font-size:11.0pt"><span style="color:black"> obesity and diabetes.</span></span><span lang="EN" style="font-size:11.0pt"></span></span></span></span></span></div> دیابت, لیراگلوتاید, موش, چاقی, بیضه Diabetes, Liraglutide, Mice, Obesity, Testis 150 160 http://journal.bums.ac.ir/browse.php?a_code=A-10-3624-1&slc_lang=fa&sid=1 Saied Mohammadzadeh سعید محمدزاده Mohammadzadeh.s@lu.ac.ir 8700319475328460049184 8700319475328460049184 Yes Department of Animal Science, Faculty of Agriculture, Lorestan University, Khoramabad, Iran گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران Mosayeb Amiri مسیب امیری Mosaiebamiri@yahoo.com 8700319475328460049185 8700319475328460049185 No Department of Animal Science, Faculty of Agriculture, Lorestan University, Khoramabad, Iran گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران