Journal of Translational Medical Research
تحقیقات پزشکی ترجمانی
J Transl Med Res.
Medical Sciences
http://journal.bums.ac.ir
87
journal87
3115-9176
3092-6912
10.61882/JBUMS
fa
jalali
1402
9
1
gregorian
2023
12
1
30
3
online
1
fulltext
fa
شبکه ارتباطی میان فاکتورهای پرتوانی در سلولهای بنیادی شبه جنینی و نقش آنها در ایجاد سرطان سلولهای جنسی بیضه
Communication network between pluripotency factors in Embryonic Stem Cell-like cells and their role in testicular germ cell cancer
بیولوژی تولید مثل
Reproductive Biology
مقاله اصیل پژوهشی
Original Article
<div style="text-align: justify;"><span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">زمینه و هدف:</span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در سلولهای بنیادی با اتصال برخی از فاکتورهای پرتوانی مانند </span></span><span dir="LTR" style="font-size:9.0pt">OCT4</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:9.0pt">NANOG</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:9.0pt">KLF4</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">SOX2</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ضمن تنظیم رونویسی باعث القای پرتوانی در سلولهای بنیادی اسپرمساز و سرطانی میشوند. در مطالعه حاضر</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> علاوه بر </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">رسم شبکه پروتئین-پروتئین و نقش این فاکتورها در بروز و پیشرفت سرطان سلولهای جنسی بیضه،</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به بررسی ایمونوهیستوشیمی ژنهای نام برده شده در سلولهای شبه جنینی (</span></span><span dir="LTR" style="font-size:9.0pt">(ESC-like cells</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">میپردازیم.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">روش تحقیق:</span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در این مطالعه تجربی، برای بررسی قدرت اثر و ارتباط میان ژنهای مذکور و رسم شبکه پروتئینی از </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">پایگاه </span></span><span dir="LTR" style="font-size:9.0pt">String</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نرمافزارهای </span></span><span dir="LTR" style="font-size:9.0pt">Cytoscape</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">Gephi</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> استفاده شد، سپس</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> سلولهای بنیادی اسپرماتوگونیال موش جدا شد و پس از کشت، سلولهای </span></span><span dir="LTR" style="font-size:9.0pt">ESC-like</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به صورت دستی از آنها استخراج شد. سپس بیان </span></span><span dir="LTR" style="font-size:9.0pt">OCT4</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:9.0pt">NANOG</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:9.0pt">KLF4</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">SOX2</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در </span></span><span dir="LTR" style="font-size:9.0pt">ESC-like cells</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با روش ایمونوهیستوشیمی (</span></span><span dir="LTR" style="font-size:9.0pt"> (ICC</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بررسی شد.</span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">یافتهها:</span></b> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">طبق نتایج بیوانفورماتیک، ژنهای هدف دارای تعامل بسیار قوی با یکدیگر میباشند که سبب پیشبرد عملکرد آنها و غنی کردن مسیرهای پیام رسانی خصوصاً در سرطان میشوند.</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> همچنین نتایج بیان دائمی ژن </span></span><span dir="LTR" style="font-size:9.0pt">OCT4</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و بیان ژنهای </span></span><span dir="LTR" style="font-size:9.0pt">NANOG</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:9.0pt">SOX2</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">KLF4</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در </span></span><span dir="LTR" style="font-size:9.0pt">ESC-like cells</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> را نشان داد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">نتیجهگیری:</span></span></b><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> این دادهها اطلاعات بیشتری در مورد پتانسیل پرتوانی </span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">ESC-like cells</span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ارائه میدهد. با توجه به ارتباط شبکهای بسیار قوی میان ژنهای مذکور و نقش آنها در غنی کردن مسیرهای سرطان و همچنین نقش کلیدی در پیشبرد اسپرماتوژنز برای درمان ناباروری در مردان و تشخیص سرطان حائز اهمیت میباشند. این یافتهها به درک بهتر کاربردهای درمانی بالقوه این ژنها کمک میکنند و راه هایی را برای تحقیقات بیشتر در این زمینهها باز میکنند.</span></span><br>
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*<b> مسئول مکاتبات:</b> <b><span dir="RTL" lang="FA" style="font-size:10.0pt"><span b="" style="font-family:" zar="">حسین عزیزی</span></span></b><br>
پست الکترونیکی: h.azizi@ausmt.ac.ir <p dir="ltr"></p>
<p dir="ltr"><a href="http://orcid.org/0000-0001-8246-595X" title="View ORCID iD Profile"><strong>View ORCID iD Profile</strong></a></p>
<p dir="ltr"><strong>You can also search for this author in:</strong> <a href="https://pubmed.ncbi.nlm.nih.gov/?term=Hossein+Azizi%2C+Amol+University+of+Special+Modern+Technologies" title="PubMed">PubMed</a> <a href="https://www.researchgate.net/profile/Hossein-Azizi-3" title="ResearchGate">ResearchGate</a> <a href="https://www.webofscience.com/wos/author/record/1854832" title="Clarivate Web of Science">S</a><a href="https://www.scopus.com">copus</a> <a href="https://scholar.google.com/" title="Google Scholar"> Google Scholar</a> <a href="https://scholar.google.com/citations?user=MhJkbC4AAAAJ&hl=en">Google Scholar Profile</a></p>
<p dir="ltr"></p></div>
<div style="text-align: justify;"><span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims:</span></b><span style="font-size:11.0pt"> In stem cells, the activation of specific powerful factors, such as <i>OCT4, NANOG, KLF4,</i> and <i>SOX2,</i> in conjunction with transcriptional control, triggers potency in both sperm-producing and cancerous stem cells. In the present study, we not only construct a protein-protein network and examine the roles of these factors in the development and advancement of testicular germ cell cancer but also explore the immunohistochemistry of the mentioned genes in pseudo-embryonic stem cells (ESC-like cells).</span><span style="font-size:11.0pt"></span></span></span><br>
<span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods:</span></b><span style="font-size:11.0pt"> In this experimental study, the String database was used along with software tools, such as Cytoscape and Gephi, to examine the strength and interaction between these genes and construct functional networks. Following this, spermatogonial stem cells were isolated from mice, and after culture, ESC-like cells were manually generated from them. Subsequently, the expression of <i>OCT4, NANOG, KLF4,</i> and <i>SOX2</i> in these ESC-like cells was investigated using immunocytochemistry (ICC).</span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b><span style="font-size:11.0pt"> According to the bioinformatics results, the target genes exhibit very strong interactions with each other, leading to the enhancement of their functionality and the enrichment of signaling pathways, particularly in cancer. Furthermore, the permanent expression of the <i>OCT4 </i>gene and the expression of the <i>NANOG, SOX2</i>, and <i>KLF4</i> genes were demonstrated in ESC-like cells.</span></span></span></span></span><br>
<b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""> These data provide further insights into the potential of ESC-like cells. Given the highly interconnected network among these mentioned genes, their roles in enriching cancer pathways, and their key role in advancing spermatogenesis for male infertility treatment and cancer diagnosis, they have significant importance. These findings contribute to a better understanding of the potential therapeutic applications of these genes and open avenues for further research in these areas.</span></span><br>
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<strong><sup>*</sup>Corresponding Author:</strong> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Hossein Azizi</span></span>; <strong>Emails:</strong> h.azizi@ausmt.ac.ir
<p><a href="http://orcid.org/0000-0001-8246-595X" title="View ORCID iD Profile"><strong>View ORCID iD Profile</strong></a></p>
<p><strong>You can also search for this author in:</strong> <a href="https://pubmed.ncbi.nlm.nih.gov/?term=Hossein+Azizi%2C+Amol+University+of+Special+Modern+Technologies" title="PubMed">PubMed</a> <a href="https://www.researchgate.net/profile/Hossein-Azizi-3" title="ResearchGate">ResearchGate</a> <a href="https://www.webofscience.com/wos/author/record/1854832" title="Clarivate Web of Science">S</a><a href="https://www.scopus.com">copus</a> <a href="https://scholar.google.com/" title="Google Scholar"> Google Scholar</a> <a href="https://scholar.google.com/citations?user=MhJkbC4AAAAJ&hl=en">Google Scholar Profile</a></p>
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سلولهای بنیادی شبه جنینی, شبکه ارتباط پروتئین-پروتئین, مسیرهای پیام رسانی, سرطان سلولهای جنسی بیضه, فاکتورهای رونویسی
Embryonic stem cell-like cells, Protein-protein interaction network, Signaling pathways, Testicular germ cells cancer, Transcription factors
216
230
http://journal.bums.ac.ir/browse.php?a_code=A-10-3523-1&slc_lang=fa&sid=1
Mahla
Masoudi
مهلا
مسعودی
mahlamasoudii@gmail.com