fa بررسی میزان بیان lncRNA DSCR9 در بیماران مبتلا به سرطان کولورکتال بيوشيمي Biochemistry مقاله اصیل پژوهشی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">زمینه و هدف:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بررسی&shy;‌ها نشان داده است که </span></span><i><span dir="LTR" style="font-size:9.0pt">DSCR</span></i> <span dir="LTR" style="font-size:9.0pt">lncRNA</span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">از سرطان و التهاب جلوگیری می&shy;‌کند و بیان آن منجر به فعالیت‌های ضد توموری می&shy;‌شود. بنابراین هدف از این تحقیق بررسی ارتباط میزان بیان </span></span><span dir="LTR" style="font-size:9.0pt">lncRNA <i>DSCR9</i></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> با خطر ابتلا به سرطان روده بزرگ بود.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">روش تحقیق:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">از </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">20 </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نمونه بافت بیماران مبتلا به سرطان کولورکتال </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و 20 بافت </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">سالم روده افراد سالم نمونه‌گیری انجام شد. </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">استخراج </span></span><span dir="LTR" style="font-size:9.0pt">Total RNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و سپس سنتز </span></span><span dir="LTR" style="font-size:9.0pt">cDNA</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انجام شد. </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بررسی بیان </span></span><span dir="LTR" style="font-size:9.0pt">lncRNA <i>DSCR9</i></span><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با استفاده از روش </span></span><span dir="LTR" style="font-size:9.0pt">Real time RT PCR</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و پرایمرهای طراحی شده با استفاده از نرم‌افزار </span></span><span dir="LTR" style="font-size:9.0pt">Beacon Designer</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> انجام شد. میزان بیان نسبی </span></span><span dir="LTR" style="font-size:9.0pt">lncRNA <i>DSCR9</i></span><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">محاسبه شد و در نهایت توسط نرم‌&shy;افزار </span></span><span dir="LTR" style="font-size:9.0pt">GraphPad Prism</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نتایج مورد تحلیل قرار گرفت</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">یافته‌ها:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نتایج نشان داد که بیان </span></span><i><span dir="LTR" style="font-size:9.0pt">DSCR9</span></i> <span dir="LTR" style="font-size:9.0pt">lncRNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در سرطان کولورکتال در مقایسه با گروه کنترل و همچنین در تومور افراد دارای متاستاز و فاقد متاستاز افزایش یافت. از طرفی بیان نسبی </span></span>&nbsp;<i><span dir="LTR" style="font-size:9.0pt">DSCR9</span></i> <span dir="LTR" style="font-size:9.0pt">lncRNA</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در تومور افراد دارای مرحله 1 و 2 و در افراد دارای تومور با اندازه متفاوت افزایش معناداری را نشان نداد؛ اما بیان نسبی </span></span><span dir="LTR" style="font-size:9.0pt">lncRNA <i>DSCR9</i></span><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در تومور افراد دارای گرید 1 و 2 افزایش بیان داشت و از نظر آماری معنا‌دار بود (0/0144</span></span><span dir="LTR" style="font-size:9.0pt"><strong>P</strong>=</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">). بررسی اختصاصیت و حساسیت </span></span><span dir="LTR" style="font-size:9.0pt">lncRNA <i>DSCR9</i></span><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نشان داد که به‌عنوان یک نشانگر (0/0001</span></span><span dir="LTR" style="font-size:9.0pt"><strong>P</strong>=</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) می‌تواند جمعیت بیمار را از سالم جدا کند.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="AR-SA" mitra="" style="font-family:">نتیجه‌گیری:</span></b> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در تحقیق حاضر بیان نسبی </span></span><span dir="LTR" style="font-size:9.0pt">lncRNA<i> DSCR9</i></span><i> </i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در افراد مبتلا به سرطان کولورکتال در مقایسه با کنترل افزایش بیان داشت که احتمالاً می&shy;تواند به دلیل فعال کردن پروتئین&shy;های دخیل در سرطان و افزایش مسیر سیگنالینگ </span></span><span dir="LTR" style="font-size:9.0pt">Wnt/beta-catenin</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> باشد و باعث رشد سلول&shy;‌های تومور و رگ‌&shy;زایی در سرطان باشد. بنابراین </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با توجه به تغییرات بیان ژن </span></span><i><span dir="LTR" style="font-size:9.0pt">DSCR9</span></i> <span dir="LTR" style="font-size:9.0pt">lncRNA<i> </i></span><i>&nbsp;</i><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در سرطان کولورکتال احتمالاً می&shy;‌توان از این ژن به عنوان یک نشانگر زیستی برای تشخیص تومور استفاده نمود.</span></span></span></span></span></span><br> <br> *<b>&nbsp;مسئول مکاتبات:</b>&nbsp;<strong>نوشا ضیاء جهرمی&nbsp;</strong><br> پست الکترونیکی:&nbsp;<span style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Nooshazia.59@gmail.com</span></span></div> <div dir="ltr" style="text-align: justify;"> <p><a href="http://orcid.org/0000-0002-6114-7060" title="View ORCID iD Profile"><strong>View ORCID iD Profile</strong></a></p> <p><strong>You can also search for this author in:</strong>&nbsp;&nbsp;<a href="https://pubmed.ncbi.nlm.nih.gov/?term=Zia-Jahromi%2C+Noosha" title="PubMed">PubMed</a>&nbsp;&nbsp; &nbsp;&nbsp;<a href="https://www.researchgate.net/profile/Noosha-Zia-Jahromi" title="ResearchGate">ResearchGate</a>&nbsp;&nbsp;&nbsp;<a href="https://www.webofscience.com/wos/author/record/1854832" title="Clarivate Web of Science">S</a><a href="https://www.scopus.com/authid/detail.uri?authorId=35319349300">copus</a>&nbsp;&nbsp;&nbsp;<a href="https://scholar.google.com/" title="Google Scholar">&nbsp;Google Scholar</a>&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://scholar.google.com/">Google Scholar Profile</a></p> </div> <div style="text-align: justify;"><span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims:</span></b> <span lang="EN" style="font-size:11.0pt">Studies have shown that lncRNA <i>DSCR9</i> prevents cancer and inflammation and its expression leads to anti-tumor activity. Therefore, this study aimed to investigate the relationship between lncRNA <i>DSCR9</i> expression and the risk of colon cancer.</span><span style="font-size:11.0pt"></span></span></span><br> <span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods:</span></b><span style="font-size:11.0pt"> Samples were taken from 20 tissues of patients with colorectal cancer and 20 healthy intestinal tissues of healthy individuals. Total RNA extraction was performed, followed by cDNA synthesis. Expression of lncRNA <i>DSCR9</i> was investigated using real-time reverse transcription&ndash;polymerase chain reaction and primers designed using Beacon Designer software. The relative expression of lncRNA <i>DSCR9</i> was calculated, and finally, the results were analyzed by GraphPad Prism software.</span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b> <span lang="EN" style="font-size:11.0pt">The results showed that lncRNA <i>DSCR9</i> expression increased in colorectal cancer compared to the control group as well as in tumors of metastatic and non-metastatic individuals. However, the relative expression of lncRNA <i>DSCR9</i> in tumors of individuals with stages 1 and 2 and in tumors of </span><span style="font-size:11.0pt">with </span><span lang="EN" style="font-size:11.0pt">different sizes did not show a significant increase; nevertheless, the relative expression of lncRNA <i>DSCR9</i> in tumors of grades 1 and 2 was statistically significant (P=0.0144). The specificity and sensitivity of lncRNA <i>DSCR9</i> showed that as a marker (P=0.0001), it could isolate the patient population from the healthy one.</span><span style="font-size:11.0pt"></span></span></span></span></span><br> <b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times=""> In the present study, there was a relative increase in the expression of lncRNA <i>DSCR9</i> in patients with colorectal cancer compared to control, which possibly could be due to the activation of proteins involved in cancer and increased Wnt/beta-catenin signaling pathway and cause tumor cell growth and angiogenesis in cancer. Therefore, due to changes in the expression of the lncRNA <i>DSCR9</i> gene in colorectal cancer, this gene may be used as a biomarker for the detection of tumors.</span></span><br> &nbsp; <p><strong>^*Corresponding Author:</strong>&nbsp;<span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Noosha Zia Jahromi</span></span>;&nbsp;<strong>Emails:</strong>&nbsp;<span style="font-size:8.0pt"><span new="" roman="" style="font-family:" times="">Nooshazia.59@gmail.com</span></span></p> <p><a href="http://orcid.org/0000-0002-6114-7060" title="View ORCID iD Profile"><strong>View ORCID iD Profile</strong></a></p> <p><strong>You can also search for this author in:</strong>&nbsp;&nbsp;<a href="https://pubmed.ncbi.nlm.nih.gov/?term=Zia-Jahromi%2C+Noosha" title="PubMed">PubMed</a>&nbsp;&nbsp; &nbsp;&nbsp;<a href="https://www.researchgate.net/profile/Noosha-Zia-Jahromi" title="ResearchGate">ResearchGate</a>&nbsp;&nbsp;&nbsp;<a href="https://www.webofscience.com/wos/author/record/1854832" title="Clarivate Web of Science">S</a><a href="https://www.scopus.com/authid/detail.uri?authorId=35319349300">copus</a>&nbsp;&nbsp;&nbsp;<a href="https://scholar.google.com/" title="Google Scholar">&nbsp;Google Scholar</a>&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://scholar.google.com/">Google Scholar Profile</a></p> <span style="font-size:10pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></b></span></span></span></div> <span style="font-size:10pt"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></b></span></span></span> نشانگر زیستی, سرطان کولورکتال,lncRNA , ژن DSCR9 Biomarker, Colorectal cancer, lncRNA, DSCR9 gene 131 140 http://journal.bums.ac.ir/browse.php?a_code=A-10-3034-4&slc_lang=fa&sid=1 Mehrnoosh Davoodi مهرنوش داودی davudimehrnoosh74@gmail.com 8700319475328460048623 0009-0008-5985-5173 No Department of Biology, Sciences Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران Noosha Zia Jahromi نوشا ضیاء جهرمی Nooshazia.59@gmail.com 8700319475328460048624 8700319475328460048624 Yes Department of Biology, Sciences Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران