fa بررسی اثر محافظتی عصاره هیدروالکلی Phlomis cancellata در مسمومیت حاد کبدی ناشی از استامینوفن در موش‌های صحرایی نر فيزيولوژي Physiology مقاله اصیل پژوهشی Original Article <div style="text-align: justify;"><span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">زمینه و هدف:</span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> استامینوفن</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">یک</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داروی</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">متداول</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">ضد</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">درد</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و ضد</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تب</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">است</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">که</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دوزهای</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بالا</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">منجر</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نکروز</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کبدی</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کلیوی در</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">انسان</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">حیوان</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">می‌گردد.</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گیاه</span></span><i><span dir="LTR" style="font-size:10.0pt">Phlomis cancellata </span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، از خانواده نعناییان،</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">دارای</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">خواص</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">آنتی‌اکسیدانی</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">است. در</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">تحقیق حاضر، </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">اثر محافظت کبدی عصاره هیدروالکلی </span></span><i><span dir="LTR" style="font-size:10.0pt">Phlomis cancellata</span></i> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در مسمومیت کبدی ناشی از استامینوفن در رت بررسی شده است.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">روش تحقیق:</span></span></b><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> در این مطالعه 36 رت</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">نر،</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">6</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گروه </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">6 تایی</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شامل: گروه</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">کنترل، گروه کنترل منفی، گروه کنترل مثبت (دریافت‌کننده سیلیمارین با دوز </span></span><span dir="LTR" style="font-size:10.0pt">mg/kg</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">200)</span></span><span dir="LTR" style="font-size:9.0pt">.</span><span style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> به تمام گروه‌ها به جز کنترل، استامینوفن با دوز 400 </span></span>&nbsp;<span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">میلی</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">گرم</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">برکیلوگرم</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">وزن</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بدن</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">داده</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">شدو گروه‌های </span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">درمان</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> 1، 2 و 3 به‌ترتیب (دوز های </span></span><span dir="LTR" style="font-size:10.0pt">mg/kg</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">200، 500 و 1000) از عصاره هیدروالکلی </span></span><i><span dir="LTR" style="font-size:10.0pt">Phlomis cancellata</span></i> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">را دریافت کردند. پس از 24</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> ساعت</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، تمام رت‌ها بی‌هوش شده و از قلب جهت ارزیابی آلبومین و بیلی‌روبین و آنزیم‌های کبدی (</span></span><span dir="LTR" style="font-size:10.0pt">ALP</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">AST</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">و </span></span><span dir="LTR" style="font-size:10.0pt">ALT</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">) خون‌گیری شد.</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بخشی از کبد جهت مطالعات بافتی نمونه‌برداری شد. نتایج با استفاده</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">از نرم‌افزار </span></span><span dir="LTR" style="font-size:10.0pt">Minitab</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> نسخه16 آنالیز شدند. داده‌ها با آزمون </span></span><span dir="LTR" style="font-size:10.0pt">ANOVA</span> <span dir="LTR" style="font-size:10.0pt">one- way</span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"> و آزمون تعقیبی توکی </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">با یکدیگر مقایسه شدند</span></span><span lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">.</span></span></span></span></span></span><br> <b><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:">یافته‌ها:</span></span></b> <span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:">بر اساس نتایج، در گروه‌های درمان و کنترل مثبت میزان آنزیم‌های کبدی نسبت به گروه کنترل منفی کاهش معنی‌داری داشته است</span></span><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;</span></span>&nbsp;<span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">(</span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">0/001</span></span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">></span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">)</span></span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">.&nbsp;</span></span></span><br> <span style="font-family:Tahoma;"><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">از</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">نظر هیستوپاتولوژیک</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">نیز،</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">نکروز</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">و</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">ارتشاح</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">لنفوسیتی در گروه</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="color:black">‌</span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">های</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">دریافت</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="color:black">‌</span></span><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">کننده</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">عصاره </span></span>&nbsp;<i><span style="font-size:10.0pt"><span style="color:black">Phlomis cancellata</span></span></i> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">و</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">گروه</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">سیلیمارین</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">کاهش</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">یافته</span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="color:black">بود</span></span><span style="font-size:9.0pt"><span style="color:black">.</span></span></span><br> <b><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">نتیجه‌گیری:</span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black"> عصاره</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">هیدروالکلی</span></span></span> <i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Phlomis cancellata</span></span></span> </i><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">در</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">بهبود</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">مسمومیت حاد</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">کبدی</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">ناشی</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">از</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">استامینوفن</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">نقش محافظتی</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span b="" mitra="" style="font-family:"><span style="color:black">دارد.</span></span></span><span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:10pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims:</span></b> <span style="font-size:12.0pt">Acetaminophen is a common anti-analgesic and antipyretic medication that in high doses leads to liver and kidney necrosis in humans and animals. <i>Phlomis cancellata</i>, from the mint family, is a plant that has antioxidant properties. In the present study, the hepatoprotective effect of <i>Phlomis cancellata</i> hydroalcoholic extract on acetaminophen-induced hepatotoxicity in rats was investigated</span><span style="font-size:11.0pt">.</span><span style="font-size:11.0pt"></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods:</span></b><span style="font-size:11.0pt"> In this study, 36 male rats were divided into six groups, each including six rats: control group, negative control group, and positive control group (receiving silymarin at a dose of 200 mg/kg) and three experimental groups. All groups, except the control group, were treated with acetaminophen at a dose of 400 mg/kg body weight, and the treatment groups of 1, 2, and 3 received</span> <span style="font-size:11.0pt">a</span><span style="font-size:11.0pt"> hydroalcoholic</span><span lang="EN" style="font-size:11.0pt"> extract</span><span style="font-size:11.0pt"> of <i>P</i></span><i><span lang="EN" style="font-size:11.0pt">hlomis cancellata</span></i> <span style="font-size:11.0pt">(at 200, 500, and 1,000 mg/kg body weight), respectively. After 24 h, all rats were anesthetized and blood was drawn from the heart to examine albumin and bilirubin, and liver enzymes of (ALP, AST, and <st1:stockticker w:st="on">ALT</st1:stockticker>). Sections of the liver were removed for histological studies. The collected data was analyzed in Minitab software (version 16). The findings were compared using post hoc Tukey and one-way ANOVA tests.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b><span style="font-size:11.0pt"> The results indicated that in the treatment and positive control groups, the amount of liver enzymes decreased significantly compared to the negative control group (P<0.001). Histopathologically, necrosis and lymphocytic infiltration were reduced in the groups receiving <i>Phlomis cancellata</i> extract and silymarin.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion:</span></b><span style="font-size:11.0pt"> Hydroalcoholic extract of <i>Phlomis cancellata</i> had a protective role in improving acute acetaminophen-induced hepatotoxicity.</span></span></span></span></span></div> استامینوفن, آنتی‌اکسیدان, مسمومیت کبدی, Phlomis cancellata Acetaminophen, Antioxidant, Hepatotoxicity, Phlomis cancellata 207 217 http://journal.bums.ac.ir/browse.php?a_code=A-10-3355-1&slc_lang=fa&sid=1 Halimeh Ajam حلیمه عجم halimeajam@gmail.com 8700319475328460044657 8700319475328460044657 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Maryam Tehranipour مریم طهرانی پور maryam_tehranipour@mshdiau.ac.ir 8700319475328460044658 8700319475328460044658 Yes Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Farahnaz Molavi فرحناز مولوی Farahmolavi@gmail.com 8700319475328460044659 8700319475328460044659 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران