TY - JOUR JF - Yektaweb JO - J Birjand Univ Med Sci VL - 23 IS - 2 PY - 2016 Y1 - 2016/5/01 TI - Comparison of antioxidant properties of N- acetylcysteine and vitamins E and C on diazinon-induced oxidative stress in rat spleen TT - مقایسه خواص آنتی‌اکسیدانی N-استیل‌سیستئین و ویتامین‌های E و C بر روی استرس اکسیداتیو ناشی از دیازینون در طحال موش صحرایی N2 - Background and Aim: Organophosphate insecticides such as diazinon (DZN) can disrupt the body's antioxidant defense system. Antioxidants protect cells against oxidative stress. In the present study, antioxidant properties of N- acetylcysteine (NAC) and vitamins E and C in reducing oxidative stress caused by DZN in rat spleen were compared. Materials and Methods: In this experimental study,48 male Wistar rats were randomly divided into eight equal groups including. control ., DZN (100 mg/kg), NAC (160 mg/kg), vitamin E (150 mg/kg), vitamin C (200 mg/kg), NAC+DZN, vitamin E+DZN, and vitamin C+DZN groups. Twenty-four hours after intraperitoneal injection.the animals were anesthetized and their spleen tissues were quickly removed. After tissues’ hemogenation superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) ,lactate dehydrogenase activities, as well as glutathione (GSH) and malondialdehyde levels were determined using biochemical methods. Results: DZN increased SOD and GST activities , but it decreased CAT activity and GSH content in the spleen. Administration of the antioxidant NAC or vitamin E caused SOD and GAT improvement. Conclusion: DZN induces oxidative stress in the spleen. NAC ,through increasing the synthesis of GSH , vitamins E ,and C- by removing free radicals- reduce DZN-induced oxidative stress. Comparing the effects of these antioxidants on GSH and GST activity indicates that the antioxidant value of NAC is greater than vitamins E and C. SP - 101 EP - 109 AU - Heydari, Javad AU - Jafari, Mahvash AU - Khazaie, Saeed AD - Chemical Injuries Research Center, Department of Biochemistry, Baqiyatallah University of Medical Sciences, Tehran, Iran. KW - Diazinon KW - N-acetyl cysteine KW - Vitamins E and C KW - Oxidative stress KW - Rat spleen UR - http://journal.bums.ac.ir/article-1-2017-en.html ER -