TY - JOUR JF - Yektaweb JO - J Birjand Univ Med Sci VL - 21 IS - 1 PY - 2014 Y1 - 2014/5/01 TI - Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease TT - بررسی شیوع حذف قطعه bp4977 از ژنوم میتوکندریایی در بیماران مبتلا به زخم پپتیک N2 - Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species (ROS) in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation (OXPHOS) predisposes the deletion of 4977 bp mtDNA. The aim of the present study was to evaluate the association of 4977 bp mtDNA deletion with peptic ulcer disease. Materials and Methods: In this case-control study included 110 patients with peptic ulcer disease and 110 healthy individuals were compared. Genomic DNAs of the cases and controls were extracted from bioptic tissues. Then, their genotypes were determined by means of Polymerase Chain Reaction (PCR). Finally, statistical analysis was performed using the MedCalc program. Results: Deletion of 4977 bp mtDNA was found to be more frequent among patients with peptic ulcer disease (52.7%) compared to the controls (15.3%). A significant association was found between the deletion with peptic ulcer disease. Conclusion: Deletion of 4977 bp in mitochondrial DNA is associated with peptic ulcer disease. SP - 48 EP - 55 AU - Haghighi, Arezoo AU - Salehi, Zivar AU - Aminian, Keyvan AU - Fakhrieh Asl, saba AD - Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran. KW - Peptic ulcer disease KW - Mitochondria genome UR - http://journal.bums.ac.ir/article-1-1438-en.html ER -