Volume 12, Issue 3 And 4 (October & January 2005)                   J Birjand Univ Med Sci. 2005, 12(3 And 4): 9-15 | Back to browse issues page

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Afzalpour M, Gharakhanlou R, Gaeini A, Seghatol Eslami A. Correlation between paraoxonase/arylesterase enzyme activities and serum lipid concentrations. J Birjand Univ Med Sci.. 2005; 12 (3 and 4) :9-15
URL: http://journal.bums.ac.ir/article-1-69-en.html
1- Assistant Professor, Department of Physical Education & Sport sciences, Faculty of Humanities, University of Birjand. Birjand, Iran , meafzalpour@yahoo.com
2- Assistant Professor, Department of Physical Education & Sport Sciences, Faculty of Humanities, University of Tarbiat Moddares. Tehran, Iran
3- Associate Professor, Department of Exercise Physiology, Faculty of Physical Education & Sport Sciences, University of Tehran. Tehran, Iran
4- Instructor; Department of Physical Education & Sport Sciences, Faculty of Humanities, University of Birjand. Birjand, Iran
Abstract:   (8846 Views)
Background and Aim: Human serum paraoxonase (PON) / aryl esterase (ARE) is synthesized by the liver and its roles are hydrolysis of organophosphate compounds in mammals and resistance against atherosclerosis. This enzyme has tree model phenotypes-distribution, including AA (homozygous with low
activity), AB (heterozygous with Average activity), or BB (homozygous with high activity). This study was aimed to measure the level of PON/ARE activity and determine the relationship between activity of this enzyme and serum lipid levels & non- lipid indices in 25-50 year-old healthy men.
Materials and Methods: Our research was a sectional and observational study. Fifty healthy men from Tarbiat Moddares University were volunteered for this research. PNO activity was measured by a spectrophotometric method using 1 ml serum; adding 1 mmol Tris/HCL buffer including 2 mmol/L calcium
chloride and 5.5 mmol/L HCL. Paranitrophenol production was measured at 405 nm wave length. ARE was determined by adding serum to 20 ml buffer including 1 mmol/L calcium chloride and 1 mmol/L phenyl acetate. Phenotype distribution was estimated by two substrates method. The correlation between PON/ARE activities and lipid & non-lipid indices were evaluated by Pearson correlation analysis. We used 2-tailed Pvalues. The P<0.05 was accepted as the level of significance.
Results: The average activities of PON and ARE were 89.14±40.62 and 100.15±36.16 U/L, respectively. This means that our participants had lower enzyme activity and all of them classified as AA phenotype. There were no significant correlations between PON/ARE activity and demographic characteristics (age,
body mass index, waist to hip ratio or blood pressure). In addition, there were no significant correlations between PON/ARE activity and lipid indices (triglyceride, total cholesterol, LDL & HDL). However, LDL/PON ratio had a significant relationship with HDL/LDL ratio(r=-0.45, P<0.002), HDL/total cholesterol ratio (r=-0.37, P<0.01), total cholesterol concentration (r=0.34, P<0.02), and LDL concentration (r=0.46,P<0.001).
Conclusion: Existence of weak correlation between serum PON/ARE activities and lipid indices along with low PON activity in studied group suggest that PON/ARE enzyme activities are under influence of genetic and racial factors.
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Type of Study: Original Article | Subject: Biochemistry
Received: 2006/09/6 | Accepted: 2014/01/8 | Published: 2014/01/8

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