Volume 28, Issue 3 (October 2021)
J Birjand Univ Med Sci. 2021, 28(3): 260-269 |
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Research code: ۹۷۰۱۵۲۶۹۹
Ethics code: IR.IAU.SHK.REC.1399.046
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Vahid P, Zia-Jahromi N. Effect of grapefruit juice on CYP2E1 gene expression in obese and control rats. J Birjand Univ Med Sci. 2021; 28 (3) :260-269
URL: http://journal.bums.ac.ir/article-1-2987-en.html
URL: http://journal.bums.ac.ir/article-1-2987-en.html
1- Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , nooshazia.59@gmail.com
2- Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , nooshazia.59@gmail.com
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ABSTRACT
1- Kamran SM, Ahmad M, Ayaz SB, Ahmad I. Frequency of non-alcoholic fatty liver disease in newly diagnosed cases of hepatitis c virus infection and its correlation with genotypes in normal weight patients. Pakistan Armed Forces Medical Journal. 2018; 68(5): 1444-48. Link
2- De Assunção SNF, Sorte NCB, Alves CD, Mendes PSA, Alves CRB, Silva LR. Nonalcoholic fatty liver disease (NAFLD) pathophysiology in obese children and adolescents: update. Nutr Hosp. 2017; 34(3): 727-30. DOI: 10.20960/nh.723
3- Golabi P, Otgonsuren M, de Avila L, Sayiner M, Rafiq N, Younossi ZM. Components of metabolic syndrome increase the risk of mortality in nonalcoholic fatty liver disease (NAFLD). Medicine (Baltimore). 2018; 97(13): 1-12. DOI: 10.1097/MD.0000000000010214
4- Alsaif FA, Alqahtani SH, Alsadoon AM, Alswat KA, Abdo AA, Hassanain MM, et al. Prevalence of biopsy-proven nonalcoholic fatty liver among patients with gallstone disease. Saudi J Gastroenterol. 2020; 26(4): 204-9. DOI: 10.4103/sjg.SJG_29_20
5- Seo Y, Aonuma K. Gamma-Glutamyl Transferase as a Risk Biomarker of Cardiovascular Disease―Does It Have Another Face? Circulation. 2017: 81(6): 783-5. CJ-17-0409. DOI: 10.1253/circj.CJ-17-0409
6- Chen J, Jiang S, Wang J, Renukuntla J, Sirimulla S, Chen J. A comprehensive review of cytochrome P450 2E1 for xenobiotic metabolism. Drug Metab Rev.. 2019; 51(2): 178-95. DOI: 10.1080/03602532.2019.1632889
7- Shahraki Mojahed L, Davari S, Hajinezhad M. The Effect of Salvia shariffi and Salvia virgata Hydroalcoholic Extracts on Some Serum Biochemical Parameters in Male Hyperlipidemic Rats. J Rafsanjan Univ Med Sci. 2017; 16(5): 437-48. [Persian] Link
8- Vyas M. Physicochemical analysis of leaves of Eriobotrya japonica and antioxidant and antidiabetic evaluation of its methanolic extract. Int J Green Pharm. 2019; 13(3): 1-10. DOI: 10.22377/ijgp.v13i3.2612
9- Ko JH, Arfuso F, Sethi G, Ahn KS. Pharmacological utilization of bergamottin, derived from grapefruits, in cancer prevention and therapy. Int J Mol Sci. 2018; 19(12): 4048. DOI: https://doi.org/10.3390/ijms19124048.
10- Holmes A, Coppey LJ, Davidson EP, Yorek MA. Rat models of diet-induced obesity and high fat/low dose streptozotocin type 2 diabetes: effect of reversal of high fat diet compared to treatment with enalapril or menhaden oil on glucose utilization and neuropathic endpoints. J Diabetes Res. 2015; 2015 307285. DOI: 10.1155/2015/307285
11- Igual M, Contreras C, Camacho M, Martínez-Navarrete N. Effect of thermal treatment and storage conditions on the physical and sensory properties of grapefruit juice. Food Bioprocess Technol. 2014; 7(1): 191-203. DOI: 10.1007/s11947-013-1088-6
12- Sani AM, Hedayati G, Arianfar A. Effect of temperature and concentration on density and rheological properties of melon (Cucumis melo L. var. Inodorus) juice. Nutr Food Sci. 2014; 44(2): 168-78. DOI: 10.1108/NFS-06-2013-0065
13- Butura A, Nilsson K, Morgan K, Morgan TR, French SW, Johansson I, et al. The impact of CYP2E1 on the development of alcoholic liver disease as studied in a transgenic mouse model. J Hepatol. 2009; 50(3): 572-83. DOI: 10.1016/j.jhep.2008.10.020
14- Khorrami M, Efati M, Zaree Mahmoudabadi A, Raouf Sarshoori J. Therapeutic Effect OF HESA-A (Herbal-Marine) on Non-alcoholic Fatty Liver Disease in Rats. J Mazandaran Univ Med Sci. 2016; 26(142): 14-22. [Persian] Link
15- Wu D, Wang X, Zhou R, Yang L, Cederbaum AI. Alcohol steatosis and cytotoxicity: the role of cytochrome P4502E1 and autophagy. Free Radic Biol Med. 2012; 53(6): 1346-57. DOI: 10.1016/j.freeradbiomed.2012.07.005
16- de la Garza AL, Etxeberria U, Haslberger A, Aumueller E, Martínez JA, Milagro FI. Helichrysum and grapefruit extracts boost weight loss in overweight rats reducing inflammation. J Med Food. 2015; 18(8): 890-8. DOI: 10.1089/jmf.2014.0088
17- Chen K, Guo R, Wei C. Synonymous mutation rs2515641 affects CYP2E1 mRNA and protein expression and susceptibility to drug-induced liver injury. Pharmacogenomics. 2020; 21(7): 459-70. DOI: 10.2217/pgs-2019-0151
18- Katary M, Abdel-Rahman AA. Alcohol suppresses cardiovascular diurnal variations in male normotensive rats: Role of reduced PER2 expression and CYP2E1 hyperactivity in the heart. Alcohol. 2020; 89: 27-36. DOI: 10.1016/j.alcohol.2020.08.001
19- Angireddy R, Chowdhury AR, Zielonka J, Ruthel G, Kalyanaraman B, Avadhani NG. Alcohol-induced CYP2E1, mitochondrial dynamics and retrograde signaling in human hepatic 3D organoids. Free Radic Biol Med. 2020; 159: 1-14. DOI: 10.1016/j.freeradbiomed.2020.06.030
20- Chudnovskiy R, Thompson A, Tharp K, Hellerstein M, Napoli JL, Stahl A. Consumption of clarified grapefruit juice ameliorates high-fat diet induced insulin resistance and weight gain in mice. PLoS One. 2014; 9(10): e108408. DOI: 10.1371/journal.pone.0108408
Full-Text: (600 Views)
ABSTRACT
Background and Aims: The CYP2E1 gene encodes cytochrome P450 enzymes that play an essential role in liver fat metabolism. Additionally, grapefruit reduces plasma lipids in the body. Therefore, herbal medicines can be considered as an important treatment strategy. The present study aimed to evaluate the effect of grapefruit juice on CYP2E1 gene expression in obese and control rats.
Materials and Methods: This experimental study was performed on 24 male Wistar rats weighing 180±20 g. Rats were divided into four groups: control (no treatment), high-fat diet group, treatment group 1 (high cholesterol diet with grapefruit juice 4 ml/kg), and treatment group 2 (high-fat diet with grapefruit juice) (8 ml/kg). They also gavaged for 6 weeks and CYP2E1 gene expression was finally determined. Statistical analyzes were performed using SPSS software (version 22).
Results: The results of CYP2E1 gene expression indicated that grapefruit juice at a dose of 8 ml/kg can further reduce the expression of CYP2E1 gene in rats with fatty liver (1.09 ±0.038) than the dose of 4 ml/kg (1.27 ±0.24). This reduction in expression was statistically significant compared to that of the high-fat diet group (3.61 ±0.25) (P=0.003).
Conclusion: The results of this study demonstrated that grapefruit juice reduces the expression of the CYP2E1 gene in obese rats due to naringin and recovers the disease by reducing the accumulation of triglycerides in the liver. Therefore, grapefruit juice can be considered as a therapeutic target in fatty liver disease and obesity.
Materials and Methods: This experimental study was performed on 24 male Wistar rats weighing 180±20 g. Rats were divided into four groups: control (no treatment), high-fat diet group, treatment group 1 (high cholesterol diet with grapefruit juice 4 ml/kg), and treatment group 2 (high-fat diet with grapefruit juice) (8 ml/kg). They also gavaged for 6 weeks and CYP2E1 gene expression was finally determined. Statistical analyzes were performed using SPSS software (version 22).
Results: The results of CYP2E1 gene expression indicated that grapefruit juice at a dose of 8 ml/kg can further reduce the expression of CYP2E1 gene in rats with fatty liver (1.09 ±0.038) than the dose of 4 ml/kg (1.27 ±0.24). This reduction in expression was statistically significant compared to that of the high-fat diet group (3.61 ±0.25) (P=0.003).
Conclusion: The results of this study demonstrated that grapefruit juice reduces the expression of the CYP2E1 gene in obese rats due to naringin and recovers the disease by reducing the accumulation of triglycerides in the liver. Therefore, grapefruit juice can be considered as a therapeutic target in fatty liver disease and obesity.
1- Kamran SM, Ahmad M, Ayaz SB, Ahmad I. Frequency of non-alcoholic fatty liver disease in newly diagnosed cases of hepatitis c virus infection and its correlation with genotypes in normal weight patients. Pakistan Armed Forces Medical Journal. 2018; 68(5): 1444-48. Link
2- De Assunção SNF, Sorte NCB, Alves CD, Mendes PSA, Alves CRB, Silva LR. Nonalcoholic fatty liver disease (NAFLD) pathophysiology in obese children and adolescents: update. Nutr Hosp. 2017; 34(3): 727-30. DOI: 10.20960/nh.723
3- Golabi P, Otgonsuren M, de Avila L, Sayiner M, Rafiq N, Younossi ZM. Components of metabolic syndrome increase the risk of mortality in nonalcoholic fatty liver disease (NAFLD). Medicine (Baltimore). 2018; 97(13): 1-12. DOI: 10.1097/MD.0000000000010214
4- Alsaif FA, Alqahtani SH, Alsadoon AM, Alswat KA, Abdo AA, Hassanain MM, et al. Prevalence of biopsy-proven nonalcoholic fatty liver among patients with gallstone disease. Saudi J Gastroenterol. 2020; 26(4): 204-9. DOI: 10.4103/sjg.SJG_29_20
5- Seo Y, Aonuma K. Gamma-Glutamyl Transferase as a Risk Biomarker of Cardiovascular Disease―Does It Have Another Face? Circulation. 2017: 81(6): 783-5. CJ-17-0409. DOI: 10.1253/circj.CJ-17-0409
6- Chen J, Jiang S, Wang J, Renukuntla J, Sirimulla S, Chen J. A comprehensive review of cytochrome P450 2E1 for xenobiotic metabolism. Drug Metab Rev.. 2019; 51(2): 178-95. DOI: 10.1080/03602532.2019.1632889
7- Shahraki Mojahed L, Davari S, Hajinezhad M. The Effect of Salvia shariffi and Salvia virgata Hydroalcoholic Extracts on Some Serum Biochemical Parameters in Male Hyperlipidemic Rats. J Rafsanjan Univ Med Sci. 2017; 16(5): 437-48. [Persian] Link
8- Vyas M. Physicochemical analysis of leaves of Eriobotrya japonica and antioxidant and antidiabetic evaluation of its methanolic extract. Int J Green Pharm. 2019; 13(3): 1-10. DOI: 10.22377/ijgp.v13i3.2612
9- Ko JH, Arfuso F, Sethi G, Ahn KS. Pharmacological utilization of bergamottin, derived from grapefruits, in cancer prevention and therapy. Int J Mol Sci. 2018; 19(12): 4048. DOI: https://doi.org/10.3390/ijms19124048.
10- Holmes A, Coppey LJ, Davidson EP, Yorek MA. Rat models of diet-induced obesity and high fat/low dose streptozotocin type 2 diabetes: effect of reversal of high fat diet compared to treatment with enalapril or menhaden oil on glucose utilization and neuropathic endpoints. J Diabetes Res. 2015; 2015 307285. DOI: 10.1155/2015/307285
11- Igual M, Contreras C, Camacho M, Martínez-Navarrete N. Effect of thermal treatment and storage conditions on the physical and sensory properties of grapefruit juice. Food Bioprocess Technol. 2014; 7(1): 191-203. DOI: 10.1007/s11947-013-1088-6
12- Sani AM, Hedayati G, Arianfar A. Effect of temperature and concentration on density and rheological properties of melon (Cucumis melo L. var. Inodorus) juice. Nutr Food Sci. 2014; 44(2): 168-78. DOI: 10.1108/NFS-06-2013-0065
13- Butura A, Nilsson K, Morgan K, Morgan TR, French SW, Johansson I, et al. The impact of CYP2E1 on the development of alcoholic liver disease as studied in a transgenic mouse model. J Hepatol. 2009; 50(3): 572-83. DOI: 10.1016/j.jhep.2008.10.020
14- Khorrami M, Efati M, Zaree Mahmoudabadi A, Raouf Sarshoori J. Therapeutic Effect OF HESA-A (Herbal-Marine) on Non-alcoholic Fatty Liver Disease in Rats. J Mazandaran Univ Med Sci. 2016; 26(142): 14-22. [Persian] Link
15- Wu D, Wang X, Zhou R, Yang L, Cederbaum AI. Alcohol steatosis and cytotoxicity: the role of cytochrome P4502E1 and autophagy. Free Radic Biol Med. 2012; 53(6): 1346-57. DOI: 10.1016/j.freeradbiomed.2012.07.005
16- de la Garza AL, Etxeberria U, Haslberger A, Aumueller E, Martínez JA, Milagro FI. Helichrysum and grapefruit extracts boost weight loss in overweight rats reducing inflammation. J Med Food. 2015; 18(8): 890-8. DOI: 10.1089/jmf.2014.0088
17- Chen K, Guo R, Wei C. Synonymous mutation rs2515641 affects CYP2E1 mRNA and protein expression and susceptibility to drug-induced liver injury. Pharmacogenomics. 2020; 21(7): 459-70. DOI: 10.2217/pgs-2019-0151
18- Katary M, Abdel-Rahman AA. Alcohol suppresses cardiovascular diurnal variations in male normotensive rats: Role of reduced PER2 expression and CYP2E1 hyperactivity in the heart. Alcohol. 2020; 89: 27-36. DOI: 10.1016/j.alcohol.2020.08.001
19- Angireddy R, Chowdhury AR, Zielonka J, Ruthel G, Kalyanaraman B, Avadhani NG. Alcohol-induced CYP2E1, mitochondrial dynamics and retrograde signaling in human hepatic 3D organoids. Free Radic Biol Med. 2020; 159: 1-14. DOI: 10.1016/j.freeradbiomed.2020.06.030
20- Chudnovskiy R, Thompson A, Tharp K, Hellerstein M, Napoli JL, Stahl A. Consumption of clarified grapefruit juice ameliorates high-fat diet induced insulin resistance and weight gain in mice. PLoS One. 2014; 9(10): e108408. DOI: 10.1371/journal.pone.0108408
Type of Study: Original Article |
Subject:
Biochemistry
Received: 2021/03/2 | Accepted: 2021/09/13 | ePublished ahead of print: 2021/09/14 | ePublished: 2021/09/18
Received: 2021/03/2 | Accepted: 2021/09/13 | ePublished ahead of print: 2021/09/14 | ePublished: 2021/09/18
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