Volume 28, Issue 3 (October 2021)
J Birjand Univ Med Sci. 2021, 28(3): 279-288 |
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Research code: ۹۷۰۲۵۷۹۶۵
Ethics code: IR.IAU.SHK.REC.1399.052
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Zandian F, Sazegar H. Comparison of the effect of exercise with Kelussia Odaratissma Mozaff on FoxO1 Expression level in cardiac tissue of obese rats. J Birjand Univ Med Sci. 2021; 28 (3) :279-288
URL: http://journal.bums.ac.ir/article-1-2951-en.html
URL: http://journal.bums.ac.ir/article-1-2951-en.html
1- Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Biotechnology Research center, Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , hoseinsazgar@yahoo.com
2- Biotechnology Research center, Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , hoseinsazgar@yahoo.com
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1- Vogelmeier CF, Criner GJ, Martinez FJ, Anzueto A, Barnes PJ, Bourbeau J, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive lung disease 2017 report. GOLD executive summary. Am J Respir Crit Care Med. 2017; 195(5): 557-82. DOI: 10.1164/rccm.201701-0218PP
2- Santilli F, D'Ardes D, Davi G. Oxidative stress in chronic vascular disease: from prediction to prevention. Vascul Pharmacol. 2015; 74: 23-37. DOI: 10.1016/j.vph.2015.09.003
3- Dighe S, Zhao J, Steffen L, Mares J, Meuer SM, Klein BE, et al. Diet patterns and the incidence of age-related macular degeneration in the Atherosclerosis Risk in Communities (ARIC) study. Br J Ophthalmol. 2020; 104(8): 1070-1076. DOI: 10.1136/bjophthalmol-2019-314813
4- Fava C, Montagnana M. Atherosclerosis is an inflammatory disease which lacks a common anti-inflammatory therapy: how human genetics can help to this issue. A narrative review. Front. Pharmacol. 2018; 9: 55. DOI: 10.3389/fphar.2018.00055
5- Förstermann U, Xia N, Li H. Roles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis. Circ Res. 2017; 120(4): 713-35. DOI: 10.1161/CIRCRESAHA.116.309326
6- Ahotupa M. Oxidized lipoprotein lipids and atherosclerosis. Free Radic Res. 2017; 51(4): 439-47. DOI: 10.1080/10715762.2017.1319944
7- Laclaustra M, Casasnovas JA, Fernández-Ortiz A, Fuster V, León-Latre M, Jiménez-Borreguero LJ, et al. Femoral and carotid subclinical atherosclerosis association with risk factors and coronary calcium: the AWHS study. J Am Coll Cardiol. 2016; 67(11): 1263-74. DOI: 10.1016/j.jacc.2015.12.056
8- Hall JE. Guyton and Hall textbook of medical physiology e-Book: Elsevier Health Sciences; 2010; 1(5): 5-8. Link
9- Xu S, Pelisek J, Jin ZG. Atherosclerosis is an epigenetic disease. Trends Endocrinol Metab. 2018; 29(11): 739-42. DOI: 10.1016/j.tem.2018.04.007
10- Dron JS, Hegele RA. Genetics of triglycerides and the risk of atherosclerosis. Curr Atheroscler Rep. 2017; 19(7): 31. DOI: 10.1007/s11883-017-0667-9
11- Kappel BA, Stöhr R, De Angelis L, Mavilio M, Menghini R, Federici M. Posttranslational modulation of FoxO1 contributes to cardiac remodeling in post-ischemic heart failure. Atherosclerosis. 2016; 249: 148-56. DOI: 10.1016/j.atherosclerosis.2016.04.001
12- Menghini R, Casagrande V, Iuliani G, Rizza S, Mavilio M, Cardellini M, et al. Metabolic aspects of cardiovascular diseases: Is FoxO1 a player or a target?Int J Biochem Cell Biol. 2020; 118: 105659. DOI: 10.1016/j.biocel.2019.105659
13- Jafarzadeh L, Habibian R, Rafieian Kopaei M, Mohammadzade Z. Effect of hydroalcoholic extract of kelussia odoratissima mozaffarian on uterus contractions of mature rats. Horizon Med Sci. 2015; 21(3): 169-74. [Persian] DOI: 10.18869/acadpub.hms.21.3.169
14- Miraj S, Jivad N, Kiani S. A review of chemical components and pharmacological effects of Kelussia odoratissima Mozaff. Der Pharmacia Lettre. 2016; 8(1): 140-7. Link
15- Sedighi M, Rafieian-Kopaei M, Noori-Ahmadabadi M. Kelussia odoratissima Mozaffarian inhibits ileum contractions through voltage dependent and beta adrenergic receptors. Life Sci J. 2012; 9(4): 1033-8. Link
16- Minaiyan M, Sajadi S, Naderi N, Taheri D. Anti-Inflammatory Effect of Kelussia odoratissima Mozaff. hydroalcoholic extract on acetic acid-induced acute colitis in rats. J Rep Pharm Sci. 2014; 3(1): 28-35. Link
17- Kurhe Y, Radhakrishnan M, Gupta D. Ondansetron attenuates depression co-morbid with obesity in obese mice subjected to chronic unpredictable mild stress; an approach using behavioral battery tests. Metab Brain Dis. 2014; 29(3): 701-10. DOI: 10.1007/s11011-014-9574-8
18- Sazegar H, Balali E, Sadeghi Samani F. Effects of Kelussia odoratissima Mozaff Hydroalcoholic Extract on Liver Injury Induced by Carbon Tetrachloride in Mice. J Ilam Univ Med Sci. 2019; 26(5): 30-41. [Persian] DOI: 10.29252/sjimu.26.5.30
19- Vester D, Lagoda A, Hoffmann D, Seitz C, Heldt S, Bettenbrock K, et al. Real-time RT-qPCR assay for the analysis of human influenza A virus transcription and replication dynamics. J Virol Methods. 2010; 168(1-2): 63-71. DOI: 10.1016/j.jviromet.2010.04.017
20- Ferns R, Nastouli E, Garson J. Quantitation of hepatitis delta virus using a single-step internally controlled real-time RT-qPCR and a full-length genomic RNA calibration standard. J Virol Methods. 2012; 179(1): 189-94. DOI: 10.1016/j.jviromet.2011.11.001
21- Ling D, Salvaterra PM. Robust RT-qPCR data normalization: validation and selection of internal reference genes during post-experimental data analysis. PloS one. 2011; 6(3): e17762. DOI: 10.1371/journal.pone.0017762
22- Ramezani M, Nasri S, Yassa N. Study of anti-inflammatory effect of aqueous and hexane extract of Apium graveolens L. in mice. Iranian Journal of Medicinal and Aromatic Plants. 2009; 24(4): 437-43. [Persian] Link
23- Garelnabi M, Mahini H. Role of polyphenolic flavonoid and exercise in atherosclerosis. Free Radic Biol Med. 2018; 128 (1): S26. DOI:10.1016/J.FREERADBIOMED.2018.10.017
24- Wang Z, Jiang X. Flavonoid-rich extract of Polygonum capitatum attenuates high-fat diet–induced atherosclerosis development and inflammatory and oxidative stress in hyperlipidemia rats. Eur J Inflamm. 2018; 16(1): 205-219. DOI: 10.1177/2058739218772710
25- Burke AC, Sutherland BG, Telford DE, Morrow MR, Sawyez CG, Edwards JY, et al. Intervention with citrus flavonoids reverses obesity and improves metabolic syndrome and atherosclerosis in obese Ldlr−/− mice. J Lipid Res. 2018; 59(9): 1714-28. DOI: 10.1194/jlr.M087387
26- Slopack D, Roudier E, Liu ST, Nwadozi E, Birot O, Haas TL. Forkhead BoxO transcription factors restrain exercise‐induced angiogenesis. J Physiol. 2014; 592(18): 4069-82. DOI: 10.1113/jphysiol.2014.275867
27- Sanchez AM. FoxO transcription factors and endurance training: a role for FoxO1 and FoxO3 in exercise-induced angiogenesis. J Physiol. 2015; 593(Pt 2): 363-4. DOI: 10.1113/jphysiol.2014.285999
28- Fouad EM, Harb OA, Amin SR. The Expression of FOXE-1 and STIP-1 in Papillary Thyroid Carcinoma and Their Relationship with Patient Prognosis. Iran J Pathol. 2018; 13(2): 256-271. Link
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ABSTRACT
Background and Aim: The FoxO1 gene is a substantial regulator of cellular metabolism in heart tissue. Therefore, the present study aimed to investigate the effect of Kelussia extract and exercise on FoxO1 expression in cardiac tissue.
Materials and Methods: In the present study, A total of 30 adult male Wistar rats weighing 180 to 200 g were selected and divided into five groups of control, negative control, a group of obese rats with exercise (rats became obese in 6 weeks and were trained 5 days a week for one hour and the extract was given to them for 6 weeks) and two treatment groups with doses of 400 and 800 mg/Kg of Kelussia extract. Molecular tests were performed on the hearts of 30 rats using Real-Time RT PCR. Finally, Statistical analysis of results was performed by SPSS software and ANOVA TEST with a significance level of P<0.05.
Results: Kelussia extract at doses of 400 and 800 mg/kg can significantly reduce FoxO1 expression level (0.61 ± 0.14) and (0.70 ± 0.25), respectively, compared to the obese group (P<0.05). Also, in the group of rats with exercise, gene expression was significantly reduced compared to that of the obese group (0.54 ± 0.13). Furthermore, the results of biochemical tests confirmed the reduction of cholesterol, sugar, LDL, and triglyceride in the hearts of obese rats treated with 400 and 800 mg/kg of Kelussia.
Conclusion: Exercise as well as Kelussia extract can reduce FoxO1 expression level and, could be used as a therapeutic target in heart disease if confirmed in further studies.
Materials and Methods: In the present study, A total of 30 adult male Wistar rats weighing 180 to 200 g were selected and divided into five groups of control, negative control, a group of obese rats with exercise (rats became obese in 6 weeks and were trained 5 days a week for one hour and the extract was given to them for 6 weeks) and two treatment groups with doses of 400 and 800 mg/Kg of Kelussia extract. Molecular tests were performed on the hearts of 30 rats using Real-Time RT PCR. Finally, Statistical analysis of results was performed by SPSS software and ANOVA TEST with a significance level of P<0.05.
Results: Kelussia extract at doses of 400 and 800 mg/kg can significantly reduce FoxO1 expression level (0.61 ± 0.14) and (0.70 ± 0.25), respectively, compared to the obese group (P<0.05). Also, in the group of rats with exercise, gene expression was significantly reduced compared to that of the obese group (0.54 ± 0.13). Furthermore, the results of biochemical tests confirmed the reduction of cholesterol, sugar, LDL, and triglyceride in the hearts of obese rats treated with 400 and 800 mg/kg of Kelussia.
Conclusion: Exercise as well as Kelussia extract can reduce FoxO1 expression level and, could be used as a therapeutic target in heart disease if confirmed in further studies.
1- Vogelmeier CF, Criner GJ, Martinez FJ, Anzueto A, Barnes PJ, Bourbeau J, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive lung disease 2017 report. GOLD executive summary. Am J Respir Crit Care Med. 2017; 195(5): 557-82. DOI: 10.1164/rccm.201701-0218PP
2- Santilli F, D'Ardes D, Davi G. Oxidative stress in chronic vascular disease: from prediction to prevention. Vascul Pharmacol. 2015; 74: 23-37. DOI: 10.1016/j.vph.2015.09.003
3- Dighe S, Zhao J, Steffen L, Mares J, Meuer SM, Klein BE, et al. Diet patterns and the incidence of age-related macular degeneration in the Atherosclerosis Risk in Communities (ARIC) study. Br J Ophthalmol. 2020; 104(8): 1070-1076. DOI: 10.1136/bjophthalmol-2019-314813
4- Fava C, Montagnana M. Atherosclerosis is an inflammatory disease which lacks a common anti-inflammatory therapy: how human genetics can help to this issue. A narrative review. Front. Pharmacol. 2018; 9: 55. DOI: 10.3389/fphar.2018.00055
5- Förstermann U, Xia N, Li H. Roles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis. Circ Res. 2017; 120(4): 713-35. DOI: 10.1161/CIRCRESAHA.116.309326
6- Ahotupa M. Oxidized lipoprotein lipids and atherosclerosis. Free Radic Res. 2017; 51(4): 439-47. DOI: 10.1080/10715762.2017.1319944
7- Laclaustra M, Casasnovas JA, Fernández-Ortiz A, Fuster V, León-Latre M, Jiménez-Borreguero LJ, et al. Femoral and carotid subclinical atherosclerosis association with risk factors and coronary calcium: the AWHS study. J Am Coll Cardiol. 2016; 67(11): 1263-74. DOI: 10.1016/j.jacc.2015.12.056
8- Hall JE. Guyton and Hall textbook of medical physiology e-Book: Elsevier Health Sciences; 2010; 1(5): 5-8. Link
9- Xu S, Pelisek J, Jin ZG. Atherosclerosis is an epigenetic disease. Trends Endocrinol Metab. 2018; 29(11): 739-42. DOI: 10.1016/j.tem.2018.04.007
10- Dron JS, Hegele RA. Genetics of triglycerides and the risk of atherosclerosis. Curr Atheroscler Rep. 2017; 19(7): 31. DOI: 10.1007/s11883-017-0667-9
11- Kappel BA, Stöhr R, De Angelis L, Mavilio M, Menghini R, Federici M. Posttranslational modulation of FoxO1 contributes to cardiac remodeling in post-ischemic heart failure. Atherosclerosis. 2016; 249: 148-56. DOI: 10.1016/j.atherosclerosis.2016.04.001
12- Menghini R, Casagrande V, Iuliani G, Rizza S, Mavilio M, Cardellini M, et al. Metabolic aspects of cardiovascular diseases: Is FoxO1 a player or a target?Int J Biochem Cell Biol. 2020; 118: 105659. DOI: 10.1016/j.biocel.2019.105659
13- Jafarzadeh L, Habibian R, Rafieian Kopaei M, Mohammadzade Z. Effect of hydroalcoholic extract of kelussia odoratissima mozaffarian on uterus contractions of mature rats. Horizon Med Sci. 2015; 21(3): 169-74. [Persian] DOI: 10.18869/acadpub.hms.21.3.169
14- Miraj S, Jivad N, Kiani S. A review of chemical components and pharmacological effects of Kelussia odoratissima Mozaff. Der Pharmacia Lettre. 2016; 8(1): 140-7. Link
15- Sedighi M, Rafieian-Kopaei M, Noori-Ahmadabadi M. Kelussia odoratissima Mozaffarian inhibits ileum contractions through voltage dependent and beta adrenergic receptors. Life Sci J. 2012; 9(4): 1033-8. Link
16- Minaiyan M, Sajadi S, Naderi N, Taheri D. Anti-Inflammatory Effect of Kelussia odoratissima Mozaff. hydroalcoholic extract on acetic acid-induced acute colitis in rats. J Rep Pharm Sci. 2014; 3(1): 28-35. Link
17- Kurhe Y, Radhakrishnan M, Gupta D. Ondansetron attenuates depression co-morbid with obesity in obese mice subjected to chronic unpredictable mild stress; an approach using behavioral battery tests. Metab Brain Dis. 2014; 29(3): 701-10. DOI: 10.1007/s11011-014-9574-8
18- Sazegar H, Balali E, Sadeghi Samani F. Effects of Kelussia odoratissima Mozaff Hydroalcoholic Extract on Liver Injury Induced by Carbon Tetrachloride in Mice. J Ilam Univ Med Sci. 2019; 26(5): 30-41. [Persian] DOI: 10.29252/sjimu.26.5.30
19- Vester D, Lagoda A, Hoffmann D, Seitz C, Heldt S, Bettenbrock K, et al. Real-time RT-qPCR assay for the analysis of human influenza A virus transcription and replication dynamics. J Virol Methods. 2010; 168(1-2): 63-71. DOI: 10.1016/j.jviromet.2010.04.017
20- Ferns R, Nastouli E, Garson J. Quantitation of hepatitis delta virus using a single-step internally controlled real-time RT-qPCR and a full-length genomic RNA calibration standard. J Virol Methods. 2012; 179(1): 189-94. DOI: 10.1016/j.jviromet.2011.11.001
21- Ling D, Salvaterra PM. Robust RT-qPCR data normalization: validation and selection of internal reference genes during post-experimental data analysis. PloS one. 2011; 6(3): e17762. DOI: 10.1371/journal.pone.0017762
22- Ramezani M, Nasri S, Yassa N. Study of anti-inflammatory effect of aqueous and hexane extract of Apium graveolens L. in mice. Iranian Journal of Medicinal and Aromatic Plants. 2009; 24(4): 437-43. [Persian] Link
23- Garelnabi M, Mahini H. Role of polyphenolic flavonoid and exercise in atherosclerosis. Free Radic Biol Med. 2018; 128 (1): S26. DOI:10.1016/J.FREERADBIOMED.2018.10.017
24- Wang Z, Jiang X. Flavonoid-rich extract of Polygonum capitatum attenuates high-fat diet–induced atherosclerosis development and inflammatory and oxidative stress in hyperlipidemia rats. Eur J Inflamm. 2018; 16(1): 205-219. DOI: 10.1177/2058739218772710
25- Burke AC, Sutherland BG, Telford DE, Morrow MR, Sawyez CG, Edwards JY, et al. Intervention with citrus flavonoids reverses obesity and improves metabolic syndrome and atherosclerosis in obese Ldlr−/− mice. J Lipid Res. 2018; 59(9): 1714-28. DOI: 10.1194/jlr.M087387
26- Slopack D, Roudier E, Liu ST, Nwadozi E, Birot O, Haas TL. Forkhead BoxO transcription factors restrain exercise‐induced angiogenesis. J Physiol. 2014; 592(18): 4069-82. DOI: 10.1113/jphysiol.2014.275867
27- Sanchez AM. FoxO transcription factors and endurance training: a role for FoxO1 and FoxO3 in exercise-induced angiogenesis. J Physiol. 2015; 593(Pt 2): 363-4. DOI: 10.1113/jphysiol.2014.285999
28- Fouad EM, Harb OA, Amin SR. The Expression of FOXE-1 and STIP-1 in Papillary Thyroid Carcinoma and Their Relationship with Patient Prognosis. Iran J Pathol. 2018; 13(2): 256-271. Link
Type of Study: Original Article |
Subject:
Biochemistry
Received: 2020/12/10 | Accepted: 2021/09/15 | ePublished ahead of print: 2021/09/18 | ePublished: 2021/09/18
Received: 2020/12/10 | Accepted: 2021/09/15 | ePublished ahead of print: 2021/09/18 | ePublished: 2021/09/18
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