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Chamani E, Ebrahimi R, Zarban A, Hoshyar R. Comparative study of the interaction of aqueous extracts of Ephedra, Nepta and Hymenocrater with histone proteins. J Birjand Univ Med Sci.. 2017; 24
URL: http://journal.bums.ac.ir/article-1-2240-en.html

1- Birjand CardioVascular Diseases Research Center, Birjand University of Medical Sciences,Birjand,Iran
2- Member of Student Research Committee, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
3- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran , reyhaneh.houshyar@gmail.com
Abstract:   (802 Views)

Background and Aim: Ephedra, Nepeta, and Hymenocrater herbs have been long used in the treatment of many diseases, but its interaction with cellular components especially nuclear proteins still unknown. On the other hand in the eukaryotic cell nucleus, histone proteins play a main role in the packaging of the genetic material as chromatin. The aim of this study was to compare the interaction of aqueous extracts obtained from ephedra, Nepeta, and Hymonocarater with histone proteins.

Materials and Methods: Histone proteins type 2A was purchased from sigma and aqueous extract of Ephedra, Nepeta and Hymenocrater were prepared in the research laboratory of Birjand University of Medical sciences. Different concentrations of aqueous extracts incubated with histone proteins, then analyzed by UV-Spectroscopy and Circular dichroism.

Results: Aqueous extract of Nepeta increased maximum absorbance of histone proteins at 210 nm, but the reduction in absorbance was shown at its high concentration. The absorbance of histone proteins was also changed in the presence of the aqueous extract of Ephedra and Hymenocraterand it was reached to zero at higher concentration of Ephedra. Circular Dichroism studies demonstrated the structure of histone proteins was changed in the presence of mentioned aqueous extract; the observed effect of the aqueous extract of ephedra was higher than Nepeta and Hymenocrater.

Conclusion: The aqueous extracts of ephedra, Nepeta, Hymenocrated interacted with histone proteins and changed their structure. The effect of ephedra was higher than others.

     
Type of Study: Original Article | Subject: Biochemistry
Received: 2017/02/11 | Accepted: 2017/03/13 | Published: 2017/04/9

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