Background and Aim: This study was to evaluate the effects of different concentrations of jujube seed oil on the hair follicle in BALB/c mice.

Materials and Methods: In this experimental study, 40 male BALB/c mice (2.5 months of age) were used. A 4 cm²of the back of animal’s body shaved with wax. The mice were then divided into five groups (n=8). The first three groups received Jujube oil concentrations 5%, 10% and 15%, respectively.The fourth and fifth groups were selected as the baseand sham control groups, respectively. The animals received topical treatment of jujuba seed oil for 12 day. Base and control groups received Ocerin and water respectively. After passing 12 day, back skin of animals was shaved againby wax and the treatment was repeated again for further 12 days. At 13^th day the animals were sacrificed  and  samples  were collected and tissue processing  and staining were performed.Data were analyzed by the ANOVA and Tukey statistical tests with the 22th version of SPSS sofware.

Results: This study showed that mean number of hair follicles in the skin of 5% jujuba essence treated group(37.75±6.6), 10% jujuba (42.75±8.3), 15% jujuba (41.25±6.2) was meaningful higher than the base (18.50±3.1)and sham (15±2.1) groups. Furthermore, we  also showed  that the  number of hair papilla, fibroblast cells and collagen fibers  were increased in the  treatment groups as compared with the control groups.

Conclusion: Based on our study, Jujube essensial oil can have very positive effects on improving hair growth and can be used for hair losing clinical trial.