Volume 22, Issue 4 (January 2015)                   J Birjand Univ Med Sci 2015, 22(4): 386-391 | Back to browse issues page

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1- Food and Drug Adjutancy , Zabol University of Medical Science, Zabol, Iran.
2- Microbiology and Molecular Genetics, Department of Microbiology , Faculty of Medicine, Zabol University of Medical Science, Zabol, Iran , zahrarashki@yahoo.co.uk
Abstract:   (10815 Views)

Background and Aim: Pseudomonas aeruginosa is one of the most important causative agents of nosocomial infections that threatens many lives .. Regarding the innate and adaptive ability of the bacteria species to become resistant to many antimicrobial agents, recognition of different antibiotic resistance patterns is extremely significant in assessing the validity of the monitoring programs. Also, the pattern of genetic isolates is essential in the management of infections caused by these bacteria. The purpose of this study was to determine genetic diversity and patterns of antimicrobial resistance of P. aeruginosa isolates using RAPD-PCR.

Materials and Methods: The present study aimed at assessing the genetic diversity and antibiotic resistant pattern of P. aeruginosa isolates in the educational Zabol hospitals. Thus, antibiotic susceptibility of 100 isolates was determined applying Kirby-Bauer disk diffusion method.

Results: RAPD-PCR data revealed  a high level of polymorphism among the isolates of P. aeruginosa in Sistan. But, no association was observed between antibiotic susceptibility and genetic diversity pattern.

Conclusion: In the present study, we RAPD-PCR technique was found to be a useful means for the investigation of the genetic variation and epidemiological study among P. aeruginosa isolates collected from Sistan region.

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Type of Study: Short Communication | Subject: Microbiology
Received: 2015/03/12 | Accepted: 2016/02/23 | ePublished: 2016/05/2

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