Volume 22, Number 2 (July 2015)                   J Birjand Univ Med Sci. 2015, 22(2): 161-168 | Back to browse issues page


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Behravan M, Malekaneh M, Mesbahzadeh B, Sharifzadeh G, Namaei M H, Behniafar H et al . Microscopic isolation and characterization of free living amoebae (FLA) from surface water sources.in Birjand, the capital city of the South Khorasan. J Birjand Univ Med Sci.. 2015; 22 (2) :161-168
URL: http://journal.bums.ac.ir/article-1-1808-en.html

1- Instructor, -Genomic Research Center, School of Paramedicine, Birjand University of Medical Science, Birjand, Iran
2- Associate Professor, -Department of Biochemistry, Birjand University of Medical Science, Birjand, Iran; , mreza.behravan@Bums.ac.ir
3- Assistant Professor, Atherosclerosis and Coronary Artery Research Centre, Department of Physiology and Pharmacology, Birjand University of Medical Science, Birjand, Iran;
4- Assistant professor, member of The Research Center of Effective Social Factors on Health, faculty of Health, Birjand University of Medical Sciences, Birjand, Iran
5- Associate Professor, Hepatitis Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
6- PhD Student, Department Parasitology and Mycology, faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran;
7- PhD Student, Department Parasitology and Mycology, faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
Abstract:   (4768 Views)
Background and Aim: Free living amoebae (FLA) are amphizoic protozoa that are widely found in various environmental sources. They are known to cause serious infections in human and animal nervous systems. . The aim of the current study was to determine the presence of Acanthamoeba spp in surface water sources in Birjand city employing microscopic culture analysis based on morphology features of the amoebae. Materials and Methods: In this cross-sectional study, 50 samples of surface water sources in Birjand city. including . parks pools, public squares, waterfronts, and water stations were collected and .transferred to the laboratory and were passed through nitrocellulose filter paper. The remained elements in the filters were cultured on non-nutrient agar (NNA) with 100μl Escherichia coli suspension. After a few weeks of using morphological features,the amoeba grown were identified. Results: Out of the total of 50 samples cultured on non-nutrient agar (NNA), 19 (38%) samples.were morphologically polluted with Acanthamoeba spp,. In 2 samples (4%) a colony of Vahlkampfiidae were observed. Conclusion: The results indicated that a significant percentage of surface water sources in Birjand city was contaminated with Acanthamoeba spp. It is necessary for physicians, therefore, to take into account the diseases caused by these infectious agents. Besides, local regional health professionals should take into consideration the potential role of surface stagnant water sources in transferring these infectious agents. Placing warning signs in areas contaminated with these infectious agents seems a useful measure.
Full-Text [PDF 460 kb]   (3295 Downloads)    
Subject: Parasitology
Received: 2015/02/3 | Accepted: 2015/07/6 | Published: 2015/08/9

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